Antioxidant-like protein 1 is altered in non-pyramidal cells and expressed in astrocytes in the gerbil hippocampal CA1 region after transient forebrain ischemia.

Abstract

In the present study, we observed chronological changes of antioxidant-like protein 1 (AOP-1) in the gerbil hippocampal CA1 region after 5 min of transient forebrain ischemia using immunohistochemistry and western blot. AOP-1 was significantly altered in the CA1 region after transient ischemia. In the sham-operated group, AOP-1 immunoreactivity was detected in pyramidal and non-pyramidal cells of the CA1 region. At 30 min after ischemic insult, AOP-1 immunoreactivity and protein level was decreased in the CA1 region. At 12 h after ischemic insult, AOP-1 immunoreactivity and protein level was highest in this region. At this time, after ischemia, AOP-1 immunoreactivity in non-pyramidal cells was high compared to the sham-operated group. Based on double immunofluorescence study, AOP-1-immunoreactive neurons were identified as GABAergic, which were stained with GAD or parvalbumin. Thereafter, AOP-1 immunoreactivity and protein levels were decreased time-dependently. From 4 days after ischemic insult, AOP 1 immunoreactivity was generally expressed in astrocytes. Five days after ischemic insult, AOP-1 immunoreactivity and protein level was increased again to 1.4 folds compared to that of the sham-operated group. In brief, AOP-1 immunoreactivity was increased in GABAergic non-pyramidal cells in the hippocampal CA1 region at early time after ischemic insult and was expressed in astrocytes at late time after ischemia. This result suggests that AOP-1 may be important role in homeostasis of GABAergic neurons because these neurons are resistant to ischemic damage.

• Authors: In Koo Hwang, Li Hua, Ki-Yeon Yoo, Dae Won Kim, Tae-Cheon Kang, Soo Young Choi, Moo Ho Won, Do-Hoon Kim
• Journal: Brain research (Brain Res) Vol. 1062 Issue 1-2 Pg. 111-9 (Nov 16 2005) ISSN: 0006-8993 [Print] Netherlands
• PMID: 16256080 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neoplasm Proteins
• gamma-Aminobutyric Acid
• Peroxidases
• Peroxiredoxins

Topics

• Animals
• Astrocytes (metabolism)
• Blotting, Western
• Disease Models, Animal
• Gerbillinae
• Hippocampus (cytology, metabolism)
• Immunohistochemistry
• Ischemic Attack, Transient (metabolism)
• Male
• Neoplasm Proteins (metabolism)
• Neurons (metabolism)
• Peroxidases (metabolism)
• Peroxiredoxins
• Prosencephalon (blood supply, metabolism, pathology)
• Time Factors
• Tissue Distribution
• gamma-Aminobutyric Acid (metabolism)