Paradoxical dose- and time-dependent regulation of superoxide dismutase and antioxidant capacity by vitamin E in rat.

Abstract

BACKGROUND:

Previous data about the regulation of SOD activity as the key part of the endogenous antioxidant system by vitamin E is conflicting.

METHODS:

We investigated the effect of different nontoxic doses of vitamin E on erythrocyte SOD activity and plasma total antioxidant capacity in rats, receiving 0 (control group), 100, 300 and 600 mg vitamin E/kilogram of body weight intramuscularly twice a week over 6-weeks.

RESULTS:

We observed a linear increase in SOD activity in the first dosing group, which was significant (p<0.05) after 6th week compared to the control level. There was an increase in SOD activity at the end of 2nd and significant increase after 4th weeks, which was followed by a significant decrease at the end of 6th week in the second dosing group. In the third dosing group, there was a significant increase at the end of 2nd week and a significant decrease at the end of 4th and 6th weeks in the SOD activity. The changes in plasma antioxidant capacity were parallel to that of SOD activity with a significant and strong degree of correlation in the 4th and 6th weeks (r=0.7 and r=0.8, respectively). Serum levels of Vitamin E also increased in a time- and dose-dependent manner; the highest level was achieved in the 600 mg/kg dosing group after 6 weeks.

CONCLUSIONS:

Non-toxic doses of vitamin E at some levels can up-regulate SOD activity, but cumulative effect of the same doses can lead to attenuation of SOD activity and hence antioxidant defense.

• Authors: Abolfazl Golestani, Roghieh Rastegar, Ahmad Shariftabrizi, Shahnaz Khaghani, Seyed Mehdi Payabvash, Amirali H Salmasi, Ahmad R Dehpour, Parvin Pasalar
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 365 Issue 1-2 Pg. 153-9 (Mar 2006) ISSN: 0009-8981 [Print] Netherlands
• PMID: 16183047 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Vitamin E
• Superoxide Dismutase

Topics

• Animals
• Antioxidants (metabolism, pharmacology)
• Dose-Response Relationship, Drug
• Male
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (blood)
• Vitamin E (administration & dosage, pharmacology)