A mutant of Escherichia coli enterotoxin inducing a specific Thl-type of T cells to varicella-zoster vaccine enhances the production of IL-12 by IFNgamma-stimulated macrophages.

Abstract	A mutant of Escherichia coli enterotoxin induces specific Thl-type T cells to varicella-zoster vaccine. The mutant increased IL-12p40, TNFalpha and nitric oxide production by IFNgamma-stimulated bone marrow macrophages but cholera toxin did not. Anti-TNFalpha antibodies blocked its stimulation of IL-12p40 production but iNOS inhibitor did not. IL-12p40 and IL-12p35 production was stimulated at the level of mRNA formation by the mutant. Cholera toxin suppressed IL-12beta1 expression by spleen T cells stimulated with anti-CD3 antibodies but the mutant did not. These findings indicate that the mutant may induce Thl-type response to the vaccine through its IL-12 and TNFalpha induction by macrophages.
Authors	Toshiyasu Shimizu, Keiko Sasaki, Michio Kato, Hideyuki Arimitsu, Sadayuki Ochi, Tomomasa Yano, Keiji Oguma, Takashi Yokochi, Takao Tsuji
Journal	Vaccine (Vaccine) Vol. 24 Issue 18 Pg. 3719-26 (May 01 2006) ISSN: 0264-410X [Print] Netherlands
PMID	16115702 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Adjuvants, Immunologic Bacterial Toxins Chickenpox Vaccine Enterotoxins Escherichia coli Proteins RNA, Messenger Receptors, Interleukin Receptors, Interleukin-12 Tumor Necrosis Factor-alpha Interleukin-12 Nitric Oxide Interferon-gamma Cholera Toxin heat-labile enterotoxin, E coli
Topics	Adjuvants, Immunologic (administration & dosage) Animals Bacterial Toxins (administration & dosage, genetics, immunology) Chickenpox Vaccine (administration & dosage, immunology) Cholera Toxin (immunology) Enterotoxins (administration & dosage, genetics, immunology) Escherichia coli Escherichia coli Proteins (administration & dosage, genetics, immunology) Female Flow Cytometry Gene Expression Regulation Herpesvirus 3, Human (immunology) Interferon-gamma (immunology) Interleukin-12 (biosynthesis) Macrophages (immunology) Mice Mice, Inbred BALB C Mice, Inbred C57BL Mutation Nitric Oxide (biosynthesis) RNA, Messenger (analysis) Receptors, Interleukin (analysis) Receptors, Interleukin-12 Th1 Cells (immunology) Transcription, Genetic Tumor Necrosis Factor-alpha (biosynthesis)

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