Abstract | BACKGROUND: Activation of the coagulation system is a critical response for both the repair of tissue injury and the host defense against microbial pathogens. Activation of the coagulation cascade culminates with the generation of thrombin. In vitro studies have shown that thrombin protects gastric epithelial cells from injury. The present study was undertaken to assess in vivo the relationship between gastric intramucosal generation of thrombin and Helicobacter pylori infection. MATERIALS AND METHODS: RESULTS: The level of TAT was significantly increased in patients with Hp+ compared to Hp- and Hp c. The levels of TAT, EGF and PGE2 were higher in VacA (+) patients than in those with VacA (-). VacA induced significant expression of tissue factor in gastric epithelial cells in vitro. The gastric intramucosal level of VacA antigen was proportionally and significantly correlated with TAT, EGF and PGE2 in Hp+ patients. The level of TAT was proportionally and significantly correlated with EGF in Hp+ patients but not in Hp- and HP c patients. CONCLUSIONS: These results showed that VacA produced by H. pylori is associated with increased thrombin generation, and that thrombin may play a protective role in H. pylori-associated gastroduodenal disorders.
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Authors | Yuko Suzuki, Esteban Cesar Gabazza, Ichiro Imoto, Roger F L James, Toshiya Hirayama, Akihiro Wada, Noriyuki Horiki, Misaki Nakamura, Hiroyuki Inoue, Mikito Kuroda, Ayumi Ogura, Yukiko Taguchi, Yutaka Yano, Osamu Taguchi, Koji Suzuki, Yukihiko Adachi |
Journal | Helicobacter
(Helicobacter)
Vol. 10
Issue 4
Pg. 323-31
(Aug 2005)
ISSN: 1083-4389 [Print] England |
PMID | 16104949
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- VacA protein, Helicobacter pylori
- antithrombin III-protease complex
- Epidermal Growth Factor
- Antithrombin III
- Peptide Hydrolases
- Thrombin
- Dinoprostone
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Topics |
- Adult
- Aged
- Antithrombin III
(metabolism)
- Bacterial Proteins
(metabolism)
- Cell Line
- Dinoprostone
(metabolism)
- Epidermal Growth Factor
(metabolism)
- Female
- Gastric Mucosa
(immunology, metabolism)
- Gastrointestinal Diseases
(microbiology)
- Helicobacter Infections
(microbiology)
- Helicobacter pylori
(pathogenicity)
- Humans
- Male
- Middle Aged
- Peptide Hydrolases
(metabolism)
- Thrombin
(metabolism)
- Up-Regulation
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