Involvement of 5-lipoxygenase in spinal cord injury.

Abstract

A traumatic spinal cord injury (SCI) induces a sequelae of events which conduce biochemical and cellular alterations. Here we compare the degree of spinal cord injury caused by the application of vascular clips, in mice lacking the 5-lipoxygenase and in the corresponding wild-type mice. Biochemical, immunohistochemical and functional studies revealed respectively an increase of neutrophils infiltration, of IL-1beta, TNF-alpha immunoreactivity, apoptosis (measured by Annexin-V staining) and loss of hind legs movement in SCI operated 5-LO wild-type mice. In contrast, the degree of (1) neutrophil infiltration at different time points, (2) cytokine expression (TNF-alpha and IL-1beta), (3) histological damage, (4) apoptosis, was markedly reduced in the tissues obtained from SCI operated 5-LO deficient mice and (5) the motor recovery was ameliorated.

• Authors: Tiziana Genovese, Emanuela Mazzon, Antonietta Rossi, Rosanna Di Paola, Giuseppe Cannavò, Carmelo Muià, Concetta Crisafulli, Placido Bramanti, Lidia Sautebin, Salvatore Cuzzocrea
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 166 Issue 1-2 Pg. 55-64 (Sep 2005) ISSN: 0165-5728 [Print] Netherlands
• PMID: 16019083 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-1
• Tumor Necrosis Factor-alpha
• Arachidonate 5-Lipoxygenase

Topics

• Animals
• Apoptosis (drug effects)
• Arachidonate 5-Lipoxygenase (deficiency, metabolism)
• Interleukin-1 (metabolism)
• Mice
• Mice, Knockout
• Neutrophil Infiltration
• Severity of Illness Index
• Spinal Cord (metabolism, physiopathology)
• Spinal Cord Injuries (pathology, physiopathology)
• Tumor Necrosis Factor-alpha (metabolism)