Effects of Carolina rinse solution, dimethyl sulfoxide, and the 21-aminosteroid, U-74389G, on microvascular permeability and morphology of the equine jejunum after low-flow ischemia and reperfusion.

Abstract

OBJECTIVE:

To evaluate effects of Carolina rinse solution, dimethyl sulfoxide (DMSO), and 21-aminosteroid, U-74389G, on microvascular permeability and morphology of the equine jejunum after low-flow ischemia and reperfusion.

ANIMALS:

20 healthy adult horses.

PROCEDURE:

Under anesthesia, full-thickness biopsy specimens of a distal portion of the jejunum were obtained for baseline measurements. In addition to a control segment, 2 jejunal segments were identified as sham-operated or experimental segments. Experimental segments underwent 60 minutes of low-flow ischemia and 3.5 hours of reperfusion. Treatments were as follows: U-74389G (3 mg/kg, IV; 6 horses), DMSO (20 mg/kg, IV; 6) diluted in 1 L of saline (0.9% NaCl) solution, local perfusion (via jejunal artery) of Carolina rinse solution (0.5 mL/kg; 4), and local perfusion of lactated Ringer's solution (0.5 mL/kg; 4).

RESULTS:

Jejunal microvascular permeability was significantly lower after treatment with Carolina rinse solution or DMSO, compared with U-74389G or lactated Ringer's solution treatments. After DMSO treatment, serosal- and submucosal-layer edema was significantly increased in experimental segments, compared with control or sham-operated segments; however, edema increases were significantly less than for lactated Ringer's solution or U-74389G treatments. Significant decreases in intestinal wet weight-to-dry weight ratio were found following Carolina rinse solution or DMSO treatments, compared with lactated Ringer's solution or U-74389G treatments. Edema formation and leukocyte infiltration in jejunal segments of horses treated with lactated Ringer's solution or U-74389G were increased, compared with Carolina rinse solution or DMSO treatments.

CONCLUSIONS AND CLINICAL RELEVANCE:

Carolina rinse solution and DMSO may be protective against ischemia-reperfusion injury in the equine jejunum.

• Authors: Robin M Dabareiner, Nathaniel A White, Jack R Snyder, Bernard F Feldman, Lydia L Donaldson
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 66 Issue 3 Pg. 525-36 (Mar 2005) ISSN: 0002-9645 [Print] United States
• PMID: 15822599 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carolina rinse solution
• Pregnatrienes
• Solutions
• U 74389F
• Dimethyl Sulfoxide

Topics

• Animals
• Body Weights and Measures (veterinary)
• Capillary Permeability (drug effects)
• Dimethyl Sulfoxide (pharmacology)
• Horse Diseases (physiopathology, prevention & control)
• Horses
• Jejunum (blood supply, drug effects, ultrastructure)
• Microscopy, Electron, Transmission (veterinary)
• Pregnatrienes (pharmacology)
• Reperfusion Injury (prevention & control, veterinary)
• Solutions (pharmacology)