[RT-PCR detecting NUP98-HOX fusion gene in leukemia].

Abstract

To investigate whether there are NUP98-HOXA, NUP98-HOXB, NUP98-HOXC, NUP98-HOXD fusion genes in leukemia patients in Xinjiang, cellular total RNA was extracted from the bone marrow mononuclear cells, the formaldehyde-agarose gel electrophoresis was used to judge whether RNA was intact, the 17 RT-PCR primers were designed to amplify the predicted fusion junctions and 412 bp GAPDH was used as an internal control, NUP98-HOXA fusion genes were amplified by nested-PCR following reverse transcription. One-step PCR was performed to amplify the other predicted fusion genes. The results showed that RNA was proved to be intact and expression of GAPDH was found in every sample. However, no predicted fusion transcripts were detected in leukemia patients. In conclusion, no NUP98-HOX fusion genes were detected in the samples from Xinjiang.

• Authors: Yan Zhang, Ling Li, Bing-Zhao Wen, Ren-Yong Lin, Xu Cao, Ning Wang, Ya Seng Ha Li Da, Ming Jiang, Hao Wen, Xiao-Mei Lu, Xiao-Hui Feng, Xin Wang
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 13 Issue 1 Pg. 83-7 (Feb 2005) ISSN: 1009-2137 [Print] China
• PMID: 15748441 (Publication Type: English Abstract, Journal Article)

Chemical References

• NUP98-HOXC13 fusion protein, human
• Oncogene Proteins, Fusion

Topics

• Adult
• Bone Marrow Cells (metabolism)
• Female
• Humans
• Leukemia (blood, genetics)
• Leukocytes, Mononuclear (metabolism)
• Male
• Oncogene Proteins, Fusion (genetics)
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Transcription, Genetic