Abstract | OBJECTIVE: METHODS: CK-deficient mice (CK KO) were examined by cardiac magnetic resonance imaging (MRI) to determine left ventricular volumes, ejection fraction, and mass: ten wild-type (WT), 6 mitochondrial CK KO (Mito-CK-/-), 10 cytosolic CK KO (M-CK-/-), and 10 mice with combined KO (M/Mito-CK-/-). RESULTS: While ejection fraction was similar in all groups, there was significant LV dilatation with a approximately 30% increase in LV end-diastolic volumes in Mito-CK-/- and in M/Mito-CK-/-. Compared to WT, there was a striking 73% and 64% increase of LV mass in Mito-CK-/- and in M/Mito-CK-/- mice, respectively, but no significant increase of LV mass (+33%; p=n.s.) in M-CK-/-. Furthermore, significant re-expression of beta-MHC, a marker of myocardial hypertrophy, was found in all CK-deficient hearts. LV remodeling was investigated by MRI in hearts of 7 WT and 10 M/Mito-CK-/- mice 4 weeks postmyocardial infarction (MI). Four weeks post-LAD ligation (MI size approximately 32%), WT and M/Mito-CK-/- showed a similar degree of cardiac dysfunction, dilatation, and hypertrophy. CONCLUSION: Mito-CK-/- and M/Mito-CK-/- mice show significant LV dilatation and marked LV hypertrophy, but LV remodeling post-MI is not aggravated. CK ablation leads to substantial adaptational changes in heart.
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Authors | Matthias Nahrendorf, Matthias Spindler, Kai Hu, Lisa Bauer, Oliver Ritter, Peter Nordbeck, Thomas Quaschning, Karl-Heinz Hiller, Julie Wallis, Georg Ertl, Wolfgang R Bauer, Stefan Neubauer |
Journal | Cardiovascular research
(Cardiovasc Res)
Vol. 65
Issue 2
Pg. 419-27
(Feb 01 2005)
ISSN: 0008-6363 [Print] England |
PMID | 15639481
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Isoenzymes
- Creatine Kinase
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Topics |
- Animals
- Cardiomyopathy, Dilated
(genetics, pathology)
- Creatine Kinase
(genetics)
- Cytosol
(enzymology)
- Female
- Hypertrophy, Left Ventricular
(genetics, pathology)
- Isoenzymes
(genetics)
- Magnetic Resonance Imaging
- Male
- Mice
- Mice, Knockout
- Mitochondria, Heart
(enzymology)
- Myocardium
(enzymology, pathology)
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