Augmented release of matrix metalloproteinase-9 by PKC activation in organotypic cultures of human breast cancer and adjacent normal breast tissue and fibroadenoma.

Abstract

The organotypic culture technique and quantitative gelatin zymography were used to determine the expression of matrix metalloproteinase (MMP)-9 and MMP-2 in human breast cancer and adjacent normal breast tissue and fibroadenoma. MMP-9 and MMP2 were constitutively expressed in all cultures. The release of these two enzymes in breast cancer was higher than that in adjacent normal breast tissue and fibroadenoma. Administration of 12-o-tetradecanoyl- phorbol-13-acetate (TPA) increased the release of MMP-9 but not of MMP-2. This response was inhibited by protein kinase C (PKC) inhibitor (H7), transcription inhibitor (actinomycin D) and translation inhibitor (cycloheximide). Moreover, the increased level of MMP-9 by TPA in breast cancer was also higher than that in adjacent normal breast tissue and fibroadenoma. These phenomena were also observed in the DAG-treated culture. These findings suggested that the MMP-9 expression in the breast cancer tissue may be more sensitive for the PKC activation.

• Authors: Trang-Tiau Wu, Jen-Hsiang Tsai, Ju-Hsin Tsai, Shou-Jen Kuo, Shi-Yau Yu, Chih-Yang Huang, Hai-Yung Hsieh, Yih-Shou Hsieh, Jer-Yuh Liu
• Journal: The Chinese journal of physiology (Chin J Physiol) Vol. 47 Issue 2 Pg. 73-8 (Jun 30 2004) ISSN: 0304-4920 [Print] India
• PMID: 15481789 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Activators
• Matrix Metalloproteinase Inhibitors
• Protease Inhibitors
• Protein Synthesis Inhibitors
• Concanavalin A
• Dactinomycin
• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
• Gelatin
• Cycloheximide
• Protein Kinase C
• Matrix Metalloproteinase 9

Topics

• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine (pharmacology)
• Breast (enzymology)
• Breast Neoplasms (enzymology)
• Concanavalin A (pharmacology)
• Cycloheximide (pharmacology)
• Dactinomycin (pharmacology)
• Enzyme Activation (drug effects)
• Enzyme Activators (pharmacology)
• Female
• Fibroadenoma (enzymology)
• Gelatin (metabolism)
• Humans
• Matrix Metalloproteinase 9 (metabolism)
• Matrix Metalloproteinase Inhibitors
• Organ Culture Techniques
• Protease Inhibitors (pharmacology)
• Protein Biosynthesis (drug effects)
• Protein Kinase C (antagonists & inhibitors, metabolism)
• Protein Synthesis Inhibitors (pharmacology)
• Transcription, Genetic (drug effects)