Montelukast modulates lung CysLT(1) receptor expression and eosinophilic inflammation in asthmatic mice.

Abstract

AIM:

To determine the expressions of cysteinyl leukotriene receptors, CysLT1 and CysLT2, in airway eosinophilic inflammation of OVA-induced asthmatic mice and the modulation by montelukast, a CysLT1 receptor antagonist.

METHODS:

Asthma model was induced by chronic exposure to ovalbumin (OVA) in C57BL/6 mice. The eosinophils in bronchoalveolar lavage (BAL) fluid and lung tissues were counted, IL-5 level in BAL fluid was measured, and CysLT1 and CysLT2 receptor mRNA expressions were detected by semi-quantitative RT-PCR.

RESULTS:

Montelukast (6 mg/kg, once per day for 20 d) significantly suppressed the increased eosinophils in BAL fluid and lung tissue, and increased IL-5 level in BAL fluid in OVA challenged mice. OVA challenge increased CysLT1 but decreased CysLT2 receptor mRNA expression. Montelukast inhibited the increased CysLT1 but not the reduced CysLT2 expression after OVA challenge.

CONCLUSION:

CysLT receptors are modulated immunologically, and montelukast inhibits up-regulation of CysLT1 receptor and airway eosinophilic inflammation in asthmatic mice.

• Authors: Yan-Jun Zhang, Lei Zhang, Shao-Bin Wang, Hua-Hao Shen, Er-Qing Wei
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 25 Issue 10 Pg. 1341-6 (Oct 2004) ISSN: 1671-4083 [Print] United States
• PMID: 15456537 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2004 Acta Pharmacologica Sinica

Chemical References

• Acetates
• Anti-Asthmatic Agents
• Cyclopropanes
• Interleukin-5
• Leukotriene Antagonists
• Membrane Proteins
• Quinolines
• RNA, Messenger
• Receptors, Leukotriene
• Sulfides
• Ovalbumin
• cysteinyl leukotriene receptor 2
• leukotriene D4 receptor
• montelukast

Topics

• Acetates (pharmacology)
• Animals
• Anti-Asthmatic Agents (pharmacology)
• Asthma (chemically induced, metabolism)
• Bronchoalveolar Lavage Fluid (chemistry)
• Cyclopropanes
• Eosinophils (metabolism, pathology)
• Interleukin-5 (metabolism)
• Leukocyte Count
• Leukotriene Antagonists (pharmacology)
• Lung (metabolism)
• Male
• Membrane Proteins (antagonists & inhibitors, biosynthesis, genetics)
• Mice
• Mice, Inbred C57BL
• Ovalbumin
• Quinolines (pharmacology)
• RNA, Messenger (biosynthesis, genetics)
• Receptors, Leukotriene (biosynthesis, genetics)
• Sulfides
• Up-Regulation