Abstract | BACKGROUND: The mechanisms involved in the impaired gallbladder contractile response in peritonitis are unknown. The aim of this study was to determine the effect of peritonitis on the contraction and relaxation responses to different agonists in gallbladder smooth muscle in guinea pig. MATERIALS AND METHODS: RESULTS: There was no significant difference between the contractile responsiveness to KCl, but maximum contractile responses (E(max)) to carbachol and histamine were significantly reduced. Indomethacin, nimesulide, and DFU concentration dependently inhibited on KCl-induced contractions of gallbladder smooth muscle. E(max) values of indomethacin, nimesulide, and DFU were significantly reduced in the peritonitis group compared with controls (P < 0.05). The inhibitor effects of nimesulide and DFU were considerably similar, but inhibitor effect of indomethacin was significantly less than that measured for nimesulide and DFU in both control and peritonitis groups (P < 0.05). CONCLUSIONS: The contraction responses to carbachol and histamine and relaxation responses to COX inhibitors on gallbladder smooth muscle are significantly decreased by peritonitis. Although the mechanism of the decrease in contraction and relaxation responses in CLP-induced peritonitis is completely unknown, we speculate that impaired smooth muscle responses may be related to an alteration in the regulation of receptor/postreceptor excitation-response coupling and/or through changes on Ca(2+) influx.
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Authors | Tijen Temiz Kaya, Gokhan Koyluoglu, Baris Karadas, Turan Yildiz, Ihsan Bagcivan, Canan Ceran, Sehsuvar Gökgöz |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 120
Issue 2
Pg. 219-24
(Aug 2004)
ISSN: 0022-4804 [Print] United States |
PMID | 15234216
(Publication Type: Journal Article)
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Chemical References |
- Cholinergic Agonists
- Cyclooxygenase Inhibitors
- Histamine
- Carbachol
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Topics |
- Animals
- Carbachol
(pharmacology)
- Cecum
- Cholinergic Agonists
(pharmacology)
- Cyclooxygenase Inhibitors
(pharmacology)
- Gallbladder
(physiopathology)
- Guinea Pigs
- Histamine
(pharmacology)
- Ligation
- Male
- Muscle Contraction
(drug effects)
- Muscle Relaxation
(drug effects)
- Muscle, Smooth
(physiopathology)
- Peritonitis
(etiology, physiopathology)
- Punctures
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