Interleukin-10 limits local and body cavity inflammation during infection with muscle-stage Trichinella spiralis.

Abstract

The aim of this study was to characterize cellular responses to muscle-stage Trichinella spiralis. From its intracellular habitat in muscle, T. spiralis secretes potent glycoprotein antigens that elicit a strong systemic host immune response. Despite the magnitude and prolonged nature of this response, nurse cells are rarely destroyed by infiltrating cells. We tested the hypothesis that the anti-inflammatory cytokine interleukin-10 (IL-10) moderates cellular responses to muscle-stage parasites. Trichinella larvae colonize the diaphragm in large numbers, prompting us to evaluate regional responses in body cavities in addition to local responses in muscle. Mice deficient in IL-10 demonstrated an exaggerated inflammatory response around nurse cells and in the pleural cavity. The effect of IL-10 was most evident 20 days following muscle infection. The increased intensity of the response in IL-10-deficient mice did not affect parasite establishment or survival. Between 20 and 50 days postinfection, the inflammatory response was diminished in both wild-type and IL-10-deficient mice. Muscle infection also elicited an antibody response, characterized initially by mixed isotypes directed at somatic larval antigens and changing to an immunoglobulin G1-dominated response directed at tyvelose-bearing excreted or secreted antigens. We conclude that IL-10 limits local and regional inflammation during the early stages of muscle infection but that chronic inflammation is controlled by an IL-10-independent mechanism that is coincident with a Th2 response.

• Authors: Daniel P Beiting, Susan K Bliss, Donald H Schlafer, Victoria L Roberts, Judith A Appleton
• Journal: Infection and immunity (Infect Immun) Vol. 72 Issue 6 Pg. 3129-37 (Jun 2004) ISSN: 0019-9567 [Print] United States
• PMID: 15155614 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Interleukin-10

Topics

• Acute Disease
• Animals
• Chronic Disease
• Inflammation (immunology)
• Interleukin-10 (immunology, metabolism)
• Larva (pathogenicity)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Muscles (immunology, parasitology, physiopathology)
• Peritoneal Cavity (physiology)
• Pleural Cavity (immunology)
• Rats
• Trichinella spiralis (growth & development, immunology, pathogenicity)
• Trichinellosis (immunology, parasitology)