The susceptibility of thermally injured mice (TI-mice) to various
infections is markedly influenced by
burn-associated type 2 T cell responses, which are common with severe thermal
injuries. Previously, we have reported that
CC chemokine ligand 2/
monocyte chemoattractant protein-I (CCL2) is produced in mice within 1 day of thermal injury, and the subsequent development of
burn-associated type 2 T cell responses are triggered by this
chemokine produced early after thermal injury. In this study, influence of
norepinephrine (NE) on CCL2 production in mice early after thermal injury (TI) was investigated. Peripheral blood mononuclear cells (PBMC) and peritoneal macrophages (PMphi) from TI-mice produced CCL2, but the same cell preparations from normal mice did not. This
chemokine was not produced by PBMC and PMphi from TI-mice previously treated with
6-hydroxydopamine (6-OHDA), which destroys sympathetic nerve termini. NE production was increased in circulation of TI-mice, and treatment of TI-mice with
6-OHDA resulted in the inhibition of NE secretion. When PBMC from normal mice were treated with NE, they acquired the ability to produce CCL2. Splenic T cells from TI-mice produced
IL-4 into their culture fluids, while the
cytokine was not produced by splenic T cells from TI-mice previously treated with
6-OHDA. These results indicate that NE may have an important role on early CCL2 production and the subsequent development of
burn-associated type 2 T cell responses.