Estradiol down-regulates CD36 expression in human breast cancer cells.

Abstract

CD36 is a trans-membrane receptor that regulates apoptosis and angiogenesis in response to its ligand thrombospondin-1 (TSP-1). This study measures expression of CD36 and TSP-1 in breast cancer cell lines. Expression of TSP-1 was approximately 50-fold higher in the aggressive cell line MDA-MB-231 than in less aggressive MCF-7, BT-474, ZR-75 and T47-D cells. In contrast, MDA-MB-231 express 30 to 100-fold less CD36 than less aggressive cells. Hormone-dependent T47-D and MCF-7 cells down-regulate CD36 in response to estradiol, and anti-hormone ICI 182,780 block this effect. These results suggest that the estrogen receptors play a role in regulating CD36 expression and ICI 182,780 prevents loss of CD36 as a novel mechanism for its anti-estrogen effect in breast cancer cells.

• Authors: Ivan P Uray, Yayun Liang, Salman M Hyder
• Journal: Cancer letters (Cancer Lett) Vol. 207 Issue 1 Pg. 101-7 (Apr 15 2004) ISSN: 0304-3835 [Print] Ireland
• PMID: 15050739 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antineoplastic Agents, Hormonal
• CD36 Antigens
• Receptors, Progesterone
• Thrombospondin 1
• Fulvestrant
• Estradiol
• RNA

Topics

• Antineoplastic Agents, Hormonal (pharmacology)
• Apoptosis
• Blotting, Northern
• Breast Neoplasms (metabolism, pathology)
• CD36 Antigens (biosynthesis)
• Cell Division
• Cell Line, Tumor
• Dose-Response Relationship, Drug
• Down-Regulation
• Estradiol (analogs & derivatives, pharmacology, physiology)
• Female
• Fulvestrant
• Humans
• Neovascularization, Pathologic
• RNA (metabolism)
• Receptors, Progesterone (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Thrombospondin 1 (metabolism)
• Time Factors