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Improvement of DNA vaccine immunogenicity by a dual antigen expression system.

Abstract
This study examined whether increased antigen expression resulted in enhanced antigen-specific immune responses in the context of DNA vaccines. To increase antigen expression, two copies of antigen expression cassettes were arranged in a plasmid pDX. BALB/c mice were intramuscularly immunized with various constructs that express influenza antigens and analysed for DNA-raised immunity. The plasmid pDX that expresses two copies of the antigen gene induced stronger antigen-specific immune responses than the plasmid pGA which expresses single antigen gene. To explore the in vivo transgene expression by pDX and pGA, luciferase activity was measured in the muscles transduced with luciferase expression plasmids. The pDX expressing two copies of luciferase induced the highest luciferase activity, which corresponded to the results from vaccination. We concluded that increasing the number of antigen expression cassettes in a vaccine construct improved antigen expression in the transduced tissue, which induced stronger DNA-raised immune responses.
AuthorsShin Sasaki, Fumihiko Takeshita, Tomohiro Oikawa, Yoshitsugu Kojima, Ke-Qin Xin, Kenji Okuda, Norihisa Ishii
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 315 Issue 1 Pg. 38-43 (Feb 27 2004) ISSN: 0006-291X [Print] United States
PMID15013422 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Viral
  • Antigens, Viral
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Nucleoproteins
  • Vaccines, DNA
  • Interleukin-4
  • Interferon-gamma
  • Luciferases
Topics
  • Animals
  • Antibodies, Viral (blood)
  • Antigens, Viral (biosynthesis, genetics, immunology)
  • Female
  • Gene Expression
  • Genes, Reporter (genetics)
  • Genetic Vectors
  • Hemagglutinin Glycoproteins, Influenza Virus (genetics, immunology)
  • Injections, Intramuscular
  • Interferon-gamma (immunology)
  • Interleukin-4 (immunology)
  • Luciferases (metabolism)
  • Mice
  • Mice, Inbred BALB C
  • Nucleoproteins (genetics, immunology)
  • Orthomyxoviridae (genetics)
  • Plasmids (genetics)
  • Promoter Regions, Genetic
  • Transduction, Genetic
  • Transgenes (genetics)
  • Vaccination
  • Vaccines, DNA (biosynthesis, genetics, immunology)

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