Identification of new candidate vaccine antigens made by Streptococcus pyogenes: purification and characterization of 16 putative extracellular lipoproteins.

Abstract	Putative extracellular lipoproteins made by group A Streptococcus (GAS) are the focus of this study, which was designed to identify new candidate vaccine antigens. Bioinformatic analysis of a serotype M1 GAS strain identified 30 open-reading frames encoding putative lipoproteins. The genes encoding the mature form of 29 of these proteins were cloned, and 16 recombinant proteins were overexpressed in Escherichia coli and purified to apparent homogeneity. The genes encoding these 16 proteins were highly conserved in GAS strains for which genome sequence data are available (serotypes M1, M3, M5, M12, M18, and M28). Mice inoculated subcutaneously with GAS and humans with GAS pharyngitis and invasive infections seroconverted to most of the 16 recombinant proteins, which indicates that these lipoproteins were produced during infection. The blood of mice actively immunized with 5 of the 16 recombinant proteins had significantly (P<.05) increased growth-inhibitory activity, compared with the blood of unimmunized mice, which identified these proteins as potential new vaccine candidates.
Authors	Benfang Lei, Mengyao Liu, Gillian L Chesney, James M Musser
Journal	The Journal of infectious diseases (J Infect Dis) Vol. 189 Issue 1 Pg. 79-89 (Jan 01 2004) ISSN: 0022-1899 [Print] United States
PMID	14702157 (Publication Type: Comparative Study, Journal Article)
Chemical References	Antibodies, Bacterial Antigens, Bacterial Lipoproteins Recombinant Proteins Streptococcal Vaccines Vaccines, Synthetic
Topics	Amino Acid Sequence Animals Antibodies, Bacterial (blood) Antigens, Bacterial (biosynthesis, immunology, isolation & purification) Cloning, Molecular Disease Models, Animal Escherichia coli (metabolism) Fasciitis, Necrotizing (blood) Humans Lipoproteins (biosynthesis, immunology, isolation & purification) Mice Molecular Sequence Data Open Reading Frames Pharyngitis (blood) Recombinant Proteins (biosynthesis) Sequence Homology, Amino Acid Species Specificity Streptococcal Infections (blood, prevention & control) Streptococcal Vaccines (administration & dosage, biosynthesis, immunology) Streptococcus pyogenes (genetics, immunology) Vaccination Vaccines, Synthetic (biosynthesis, immunology)

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