Abstract |
We studied whether induction of glucose transporters (GLUTs) 1 to 4 correlates with human papillomavirus (HPV)-dependent malignant transformation of cervical epithelium. Tissue samples of cervical intraepithelial neoplasia (CIN; grades 1 to 3), invasive carcinomas, and lymph node metastasis were examined. HPV typing was performed. Tissue sections were immunostained with GLUT1 to GLUT4 antibodies. Messenger RNA ( mRNA) in situ hybridization confirmed GLUT1 protein expression. Weak expression of GLUT1 was found in nondysplastic HPV-positive and HPV-negative epithelium; significant expression was observed in preneoplastic lesions, correlating with the degree of dysplasia. In CIN 3 high-risk HPV lesions, cervical cancer, and metastasis, GLUT1 was expressed at highest levels with a strong correlation of GLUT1 mRNA and protein expression. Immunostains for GLUT2 to GLUT4 were negative. Cervical tumor cells respond to enhanced glucose utilization by up-regulation of GLUT1. The strong induction of GLUT1 mRNA and protein in HPV-positive CIN 3 lesions suggests GLUT1 overexpression as an early event in cervical neoplasia. GLUT1 is potentially relevant as a diagnostic tool and glucose metabolism as a therapeutic target in cervical cancer.
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Authors | Christian Rudlowski, Albert J Becker, Willibald Schroder, Werner Rath, Reinhard Büttner, Markus Moser |
Journal | American journal of clinical pathology
(Am J Clin Pathol)
Vol. 120
Issue 5
Pg. 691-8
(Nov 2003)
ISSN: 0002-9173 [Print] England |
PMID | 14608894
(Publication Type: Journal Article)
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Chemical References |
- Glucose Transporter Type 1
- Monosaccharide Transport Proteins
- RNA, Messenger
- SLC2A1 protein, human
- Glucose
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Topics |
- Cell Transformation, Neoplastic
- Female
- Glucose
(metabolism)
- Glucose Transporter Type 1
- Humans
- Immunohistochemistry
- Monosaccharide Transport Proteins
(analysis, genetics)
- Papillomaviridae
- Papillomavirus Infections
(complications, metabolism)
- RNA, Messenger
(analysis)
- Uterine Cervical Neoplasms
(etiology, metabolism, pathology)
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