Apoptosis regulation differs between ulcerative colitis-associated and sporadic colonic tumors. Association with survivin and bcl-2.

Abstract

To clarify kinetics in ulcerative colitis (UC)-associated lesions, cell proliferation, apoptosis, and expression of apoptosis-inhibitory proteins were studied. Ki-67 labeling and survivin and bcl-2 expression were examined immunohistochemically in 22 low-grade dysplasias (LGDs), 25 high-grade dysplasias (HGDs), and 13 adenocarcinomas associated with UC, and for comparison in 21 sporadic adenomas with LGD, 22 sporadic adenomas with HGD, and 21 invasive adenocarcinomas. Apoptosis was studied with nick-end labeling and immunohistochemical analysis of single-stranded DNA. In UC-associated LGDs, Ki-67--positive cells were more frequent in the lower than the upper half of the crypt, related to bcl-2 expression, while in sporadic adenomas such cells were more common in the upper half. No difference in apoptosis was found between UC-associated LGDs and sporadic adenomas with LGD or between UC-associated HGDs and sporadic adenomas with HGD. However, UC-associated carcinomas exhibited a lower apoptotic count than their sporadic invasive counterparts. This seemed related to higher survivin expression without a significant difference between the 2 types of invasive lesions regarding bcl-2 levels. Apoptosis is less frequent in UC-associated than in sporadic invasive colon carcinomas, this being linked to elevated survivin expression. The control of apoptosis may be different in the 2 types of tumorigenesis.

• Authors: Tetuo Mikami, Tsutomu Yoshida, Fumiyuki Akino, Tadashi Motoori, Mioko Yajima, Isao Okayasu
• Journal: American journal of clinical pathology (Am J Clin Pathol) Vol. 119 Issue 5 Pg. 723-30 (May 2003) ISSN: 0002-9173 [Print] England
• PMID: 12760292 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BIRC5 protein, human
• Inhibitor of Apoptosis Proteins
• Microtubule-Associated Proteins
• Neoplasm Proteins
• Survivin
• Cyclin D1

Topics

• Adenoma (pathology)
• Apoptosis
• Carcinoma (complications, metabolism, pathology)
• Cell Division
• Colitis, Ulcerative (complications)
• Colonic Neoplasms (etiology, metabolism, pathology)
• Cyclin D1 (metabolism)
• Humans
• Immunohistochemistry
• Inhibitor of Apoptosis Proteins
• Microtubule-Associated Proteins (metabolism)
• Neoplasm Proteins
• Survivin