[Screening and application of human-derived HBsAg bacteriophage single chain antibody in clinical diagnosis].

Abstract

OBJECTIVE:

To identify human single chain Fv antibody (ScFv) against hepatitis B viral surface antigen.

METHODS:

The recombinant phages were panned by HBsAg which was coated in a microtiter plate, after five rounds of biopanning, 56 phage clones were identified specific to HBsAg. The specificity of ScFv was evaluated by ELISA and immunohistochemistry, respectively.

RESULTS:

The data of HB sAg-ScFv DNA digestion and DNA sequencing showed that the ScFv gene is composed of 750 bp. ELISA and immunohistochemistry demonstrated that the human single chain Fv antibody against hepatitis B surface antigen has a specific combination character with hepatitis B surface antigen of different sources and paraffin-embedded patients tissue specimens, it did not react with normal liver tissue and HCV.

CONCLUSIONS:

The application of HBsAg specific ScFv in immunohistochemistry was successfully achieved.

• Authors: Yanwei Zhong, Jun Cheng, Shuangshuang Shi, Jingmin Zhao, Gang Wang, Xiaobing Xia, Xiaojun Tian, Li Li, Lingxia Zhang
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 16 Issue 3 Pg. 223-5 (Sep 2002) ISSN: 1003-9279 [Print] China
• PMID: 12665924 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hepatitis B Antibodies
• Hepatitis B Surface Antigens
• Peptide Library

Topics

• Bacteriophages (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Hepatitis B Antibodies
• Hepatitis B Surface Antigens (immunology)
• Hepatitis B, Chronic (diagnosis)
• Humans
• Peptide Library