The investigations of four Cases involving mold-contaminated buildings and human reaction to exposure, documents tests of extracted urine containing
trichothecene mycotoxins confirming exposure and the diagnosis of
mycotoxicosis in humans. In each of four Cases, the urine demonstrated
antibiotic activity,
sulfuric acid charring, and
protein release. Urine was extracted using
ethyl acetate 40V/60V[EA]. Extracted
mycotoxin spotted on (TLC) displayed color and a range of (rf) between 0.2-0.6 using various
solvents. Extract was re-suspended using 50%
ethanol V/V to inject
mycotoxins into weanling female Sprague-Dawley rats. Degeneration and
necrosis of the rat's tissue followed. Koch's Postulates conditions were fulfilled by isolation of the causative agent, the
trichothecene mycotoxins and the reproduction of disease. Examination of human tissue within the urine extraction group confirms Koch's Postulates and comparative pathology confirms inhalation
Mycotoxicosis, with severe
necrosis of the central nervous system and severe
scarring within the lungs. Extraction of
mycotoxins from human patient urine is a very useful confirmatory test to demonstrate exposure and identify
mycotoxicosis. Low concentrations (6%) of
sodium hypochlorite were ineffective against the activity of
trichothecene mycotoxin. The severity or stages of disease directly correlates the level of exposure or
poisoning (Patent Pending).