Relationship between overexpression of NK-1R, NK-2R and intestinal mucosal damage in acute necrotizing pancreatitis.

Abstract

AIM:

To study the expression of neurokinin-1 receptor (NK-1R) and neurokinin-2 receptor (NK-2R) in distal ileum of acute necrotizing pancreatitis (ANP) and to evaluate the relationship between expression of these two receptors and intestinal mucosal damage.

METHODS:

A total of 130 adult Sprague-Dawley rats were randomly divided into two groups: the rats in ANP group (n=80) were induced by the retrograde intraductal infusion of 30 g.L(-1) sodium taurocholate. And the rats in normal control group (n=50) received laparotomy only. Sacrifices were made 6 h, 12 h, 24 h and 48 h later in ANP and normal control group after induction respectively. Intestinal mucosal permeability was studied by intrajejunal injection of 1.5 mCi radioactive isotope (99m)Tc-diethlene triamine pentacetic acid (DTPA) and the radioactivity of (99m)Tc-DTPA content in urine was measured 6 h, 12 h, 24 h and 48 h after induction. Then the pancreas and intestine were prepared for pathology. Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the mRNA expression of NK-1R and NK-2R, and Western blot was used to investigate the protein level of NK-1R and NK-2R.

RESULTS:

In ANP rats, serious histologic damages in intestinal mucosa were observed, and the radioactivity of (99m)Tc-DTPA in urine increased significantly in the ANP group. RT-PCR revealed that NK-1R and NK-2R mRNA level was overexpressed in the distal ileum of ANP as compared with the normal control group. Western blot discovered stronger NK-1R (14-fold increase) and NK-2R (9-fold increase) immunoreactivity in the intestinal mucosa of ANP rats. Moreover, the overexpression of NK-1R was associated with mucosal pathological score (r=0.77, P<0.01) and intestinal permeability (r=0.68, P<0.01) in ANP rats.

CONCLUSION:

NK-1R and NK-2R contribute to disrupted neuropeptides loop balance, deteriorate intestinal damage, and are involved in pathophysiological changes in ANP.

• Authors: Xin Shi, Nai-Rong Gao, Qing-Ming Guo, Yong-Jiu Yang, Ming-Dong Huo, Hao-Lin Hu, Helmut Friess
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 9 Issue 1 Pg. 160-4 (Jan 2003) ISSN: 1007-9327 [Print] United States
• PMID: 12508374 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Receptors, Neurokinin-1
• Receptors, Neurokinin-2
• Amylases

Topics

• Amylases (blood)
• Animals
• Intestinal Mucosa (metabolism, pathology)
• Pancreatitis, Acute Necrotizing (metabolism, pathology)
• RNA, Messenger (metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Receptors, Neurokinin-1 (genetics, metabolism)
• Receptors, Neurokinin-2 (genetics, metabolism)
• Statistics as Topic