Abstract | BACKGROUND: Th2 and Th1 cells have been suggested to express CCR3/CCR4 and CCR5/CXCR3, respectively. OBJECTIVE: We examined CCR3, CCR4, CCR5 and CXCR3 expression and cytokine production in peripheral blood CD4+ T cells from patients with atopic dermatitis (AD), which has been postulated to be a Th2-type cell-mediated disease, and then analysed the possible correlation between these values and the levels of several clinical parameters. METHODS: Intracellular cytokine production and chemokine receptor expression in peripheral blood CD4+ T cells from 40 AD patients and 20 sex- and age-matched healthy control subjects were studied by flow cytometry. RESULTS: The frequencies of IL-4- and IL-13-producing CD4+ T cells from patients with AD were significantly higher than those from healthy control subjects (IL-4:3.9 +/- 2.1% vs. 1.6 +/- 0.7%, P = 0.0005, IL-13:4.0 +/- 2.1% vs. 1.8 +/- 0.8%, P = 0.0023), whereas the frequencies of IL-2- and IFN-gamma-producing CD4+ T cells were significantly decreased in AD patients (IL-2:38.1 +/- 10.3% vs. 51.3 +/- 6.3%, P = 0.0003, IFN-gamma: 9.9 +/- 3.5% vs. 26.4 +/- 4.6%, P < 0.0001). The percentage of CCR4+ cells in CD4+ CD45RO+ T cells in AD patients was significantly higher than that in healthy control subjects (24.4 +/- 8.0% vs. 10.9 +/- 2.3%, P < 0.0001) and was correlated positively with the total serum IgE, serum lactic dehydrogenase (LDH) level, eosinophil number, eruption score, and IL-4 and IL-13 secretion in CD4+ T cells, and inversely with IL-2 and IFN-gamma secretion in CD4+ T cells. In contrast, CCR3 was not detected on circulating CD4+ T cells even in AD patients. On the other hand, the percentage of CCR5+ or CXCR3+ cells in CD4+ CD45RO+ T cells in AD patients was significantly decreased (CCR5:23.2 +/- 7.0% vs. 28.4 +/- 5.4%, P = 0.023, CXCR3:29.9 +/- 11.4% vs. 38.5 +/- 6.7%, P = 0.028) and was positively correlated with eruption score (P < 0.05). Multiple regression analyses showed that the percentage of CCR4 expression highly correlated with serum IgE, LDH, eosinophil number and eruption in AD patients. CONCLUSION: CCR4+ cells might be involved in the aetiopathogenesis of AD.
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Authors | H Okazaki, M Kakurai, D Hirata, H Sato, T Kamimura, N Onai, K Matsushima, H Nakagawa, S Kano, S Minota |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 32
Issue 8
Pg. 1236-42
(Aug 2002)
ISSN: 0954-7894 [Print] England |
PMID | 12190665
(Publication Type: Journal Article)
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Chemical References |
- CCR4 protein, human
- Cytokines
- Interleukin-13
- Interleukin-2
- Receptors, CCR4
- Receptors, Chemokine
- Interleukin-4
- Immunoglobulin E
- Interferon-gamma
- L-Lactate Dehydrogenase
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Topics |
- Adolescent
- Adult
- CD4-Positive T-Lymphocytes
(immunology)
- Case-Control Studies
- Child
- Cytokines
(metabolism)
- Dermatitis, Atopic
(immunology)
- Eosinophils
(immunology)
- Flow Cytometry
- Humans
- Immunoglobulin E
(blood)
- Interferon-gamma
(metabolism)
- Interleukin-13
(metabolism)
- Interleukin-2
(metabolism)
- Interleukin-4
(metabolism)
- L-Lactate Dehydrogenase
(blood)
- Leukocyte Count
- Logistic Models
- Receptors, CCR4
- Receptors, Chemokine
(analysis)
- Th1 Cells
(immunology)
- Th2 Cells
(immunology)
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