The class IV neuronal intermediate filament (IF) family
proteins includes the neurofilament (NF) triplet
proteins NF-L, NF-M, and NF-H and also the more recently characterized alpha-internexin-NF66. It is well established that NF-L, -M, and -H
protein and
mRNA are downregulated after
peripheral nerve injury. We examined
alpha-internexin protein expression after three facial nerve lesion paradigms: crush, transection, and resection.
Alpha-internexin immunoreactivity was absent in the perikarya of uninjured facial motoneurons but increased dramatically in all three injury paradigms, with maximum immunoreactivity observed at 7 d after injury. Twenty-eight days after
nerve crush or transection, there was a dramatic decrease in the number of
alpha-internexin-positive cells. In contrast,
alpha-internexin remained elevated 28 d after nerve resection, an injury that hinders regeneration and target reinnervation. In situ hybridization studies showed an increase in
alpha-internexin mRNA expression in the facial nucleus at 7 and 14 d after injury. Retrograde transport of
fluorogold from the whisker pads to the facial nucleus was seen only in motoneurons that lacked
alpha-internexin immunoreactivity, supporting the idea that target reinnervation and inhibitory signals from the periphery regulate the expression of
alpha-internexin. Blockage of axonal transport through local
colchicine application induced strong immunoreactivity in motoneurons.
Alpha-internexin expression was also examined after central
axotomy of rubrospinal neurons, which constitutively show
alpha-internexin immunoreactivity. After rubrospinal tractotomy,
alpha-internexin immunoreactivity transiently increased by 7 d after injury but returned to control levels by 14 d. We conclude that
alpha-internexin upregulation in injured motoneurons suggests a role for this IF
protein in neuronal regeneration.