Apoptosis of a human non-small cell lung cancer (NSCLC) cell line, PLA-801, induced by acutiaporberine, a novel bisalkaloid derived from Thalictrum acutifolium (Hand.-Mazz.) Boivin.

Abstract

Acutiaporberine is a novel ether-linked bisalkaloid isolated from the traditional Chinese medicinal herb Thalictrum acutifolium (Hand.-Mazz.) Boivin (TAB). The present study demonstrates for the first time, by means of nuclear staining, DNA agarose gel electrophoresis, and flow cytometry, that acutiaporberine induces apoptosis in PLA-801 cells, a cultured human non-small cell lung cancer (NSCLC) cell line. An immunohistochemical assay and western immunoblot analysis showed down-regulation of the bcl-2 gene and up-regulation of the bax and c-myc genes in the acutiaporberine-treated cells. The observations also indicate that acutiaporberine induces apoptosis of PLA-801 cells in a concentration- and time-dependent manner. These results suggest that acutiaporberine may be a potential, natural apoptosis-inducing agent for NSCLC.

• Authors: Qi Chen, Wenlie Peng, Anlong Xu
• Journal: Biochemical pharmacology (Biochem Pharmacol) Vol. 63 Issue 8 Pg. 1389-96 (Apr 15 2002) ISSN: 0006-2952 [Print] England
• PMID: 11996879 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Antineoplastic Agents, Phytogenic
• Aporphines
• Berberine Alkaloids
• Ethers
• acutiaporberine

Topics

• Alkaloids (pharmacology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis
• Aporphines
• Berberine Alkaloids
• Blotting, Western
• Carcinoma, Non-Small-Cell Lung (pathology)
• Cell Size (drug effects)
• DNA Fragmentation (drug effects)
• Ethers (pharmacology)
• Humans
• Immunohistochemistry
• Inhibitory Concentration 50
• Lung Neoplasms (pathology)
• Ranunculaceae (chemistry)
• Tumor Cells, Cultured