Abstract | BACKGROUND: Our previous study using allergen-sensitized murine splenocyte cultures has shown that Lactobacillus casei strain Shirota (LcS), a lactic acid bacterium widely used as a starter for fermented milk products, suppresses IgE production through promoting a dominant Th1-type response mediated by IL-12 induction. OBJECTIVE: METHODS: The ability of heat-killed LcS to induce IL-12 in serum was tested. OVA-TCR-Tg mice were fed a diet containing OVA for 4 weeks and injected with LcS intraperitoneally three times in the first week of this period. Cytokine and antibody secretion by splenocytes, and serum IgE and IgG1 responses were examined. The inhibitory effect of LcS on systemic anaphylaxis induced by intravenous challenge of OVA-fed OVA-TCR-Tg mice with OVA was also tested. RESULTS:
Intraperitoneal injection of LcS induced an IL-12 response in the serum of OVA-TCR-Tg mice. In the food allergy model, LcS administration skewed the pattern of cytokine production by splenocytes toward Th1 dominance, and suppressed IgE and IgG1 secretion by splenocytes. The ability of LcS to modulate cytokine production was blocked by anti-IL-12 antibody treatment. LcS also inhibited serum OVA-specific IgE and IgG1 responses and diminished systemic anaphylaxis. CONCLUSION:
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Authors | K Shida, R Takahashi, E Iwadate, K Takamizawa, H Yasui, T Sato, S Habu, S Hachimura, S Kaminogawa |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 32
Issue 4
Pg. 563-70
(Apr 2002)
ISSN: 0954-7894 [Print] England |
PMID | 11972603
(Publication Type: Journal Article)
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Chemical References |
- Antibodies
- Cytokines
- Immunoglobulin G
- OVA 323-339
- Peptide Fragments
- Interleukin-12
- Immunoglobulin E
- Ovalbumin
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Topics |
- Anaphylaxis
(prevention & control)
- Animals
- Antibodies
(pharmacology)
- Cells, Cultured
- Cytokines
(biosynthesis)
- Food Hypersensitivity
(immunology)
- Genes, T-Cell Receptor
- Immunoglobulin E
(biosynthesis, blood)
- Immunoglobulin G
(biosynthesis, blood)
- Interleukin-12
(antagonists & inhibitors, blood, immunology)
- Lacticaseibacillus casei
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Transgenic
- Ovalbumin
(immunology)
- Peptide Fragments
(immunology)
- Spleen
(cytology, immunology)
- Th1 Cells
(immunology)
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