[Effects of IFN-alpha combined with IL-6 on cell growth and related genes expression and apoptosis of bone marrow cells from CGL patients].

Abstract

OBJECTIVE:

To investigate the effects of interferon-alpha (IFN-alpha) and IFN-alpha combined with interleukin-6 (IL-6) on cell growth and bcr/abl, bcl-2 and c-myc genes expression in the bone marrow mononuclear cells (MNC) from chronic granulocytic leukemia (CGL) patients.

METHODS:

MNCs were cultured in liquid medium at the presence of IFN-alpha (200 U/ml) or IFN-alpha (200 U/ml) plus IL-6 (100 ng/ml). The viable cells were counted and the expression levels of beta-actin, bcr/abl, bcl-2 and c-myc genes were quantitatively detected by reverse transcriptase-polymerase chain reaction (RT-PCR).

RESULTS:

The cell growth was markedly inhibited by IFN-alpha, but the extent of the inhibition was slightly decreased when IFN-alpha combined with IL-6. The expression levels of bcr/abl and bcl-2 gene were reduced by IFN-alpha or IFN-alpha plus IL-6. The expression of c-myc gene was inhibited by IFN-alpha but promoted by IL-6.

CONCLUSIONS:

Both IFN-alpha and IFN-alpha plus IL-6 can inhibit the expression of anti-apoptosis genes, and modulate the expression of c-myc. It is the possible mechanism of IFN-alpha therapy for CGL in chronic phase.

• Authors: H Chen, L Tang, X Peng, Z Luo, S Luo, W Tan
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 21 Issue 7 Pg. 341-4 (Jul 2000) ISSN: 0253-2727 [Print] China
• PMID: 11877000 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interferon-alpha
• Interleukin-6
• Proto-Oncogene Proteins c-bcl-2
• Proto-Oncogene Proteins c-myc
• RNA, Messenger
• Fusion Proteins, bcr-abl

Topics

• Adolescent
• Adult
• Apoptosis (drug effects)
• Bone Marrow Cells (drug effects, metabolism, pathology)
• Cell Count
• Cell Division (drug effects)
• Drug Interactions
• Fusion Proteins, bcr-abl (genetics)
• Gene Expression Regulation (drug effects)
• Humans
• Interferon-alpha (pharmacology)
• Interleukin-6 (pharmacology)
• Leukemia, Myelogenous, Chronic, BCR-ABL Positive (blood, genetics, pathology)
• Middle Aged
• Proto-Oncogene Proteins c-bcl-2 (genetics)
• Proto-Oncogene Proteins c-myc (genetics)
• RNA, Messenger (drug effects, genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction