Antitumor effect of an adeno-associated virus vector containing the human interferon-beta gene on experimental intracranial human glioma.

Abstract

We constructed an adeno-associated virus (AAV) vector containing the human interferon-beta (HuIFN-b ) gene (AAV-IFN-beta) and investigated its antitumor effect against human glioma cells (U251-SP) inoculated into the brain of nude mice. Prior to this, we examined human glioma cells transduced with AAV-IFN-beta using video-enhanced contrast differential interference contrast (VEC-DIC) microscopy. Infection of AAV-IFN-beta induced apoptosis and secondary necrosis in human glioma cells. In in vivo experiments, we confirmed production of HuIFN-beta and induction of heat-shock protein (HSP) in glioma cells transduced with AAV-IFN-beta. Growth of the experimental gliomas was completely inhibited by six injections of AAV-IFN-beta, starting 7 days after transplantation of glioma cells. In addition, the survival of mice treated with AAV-IFN-beta was remarkably prolonged. These results indicate that AAV-IFN-beta induces apoptosis of glioma cells and has a strong antitumor effect in this experimental glioma model.

• Authors: Jun Yoshida, Masaaki Mizuno, Norimoto Nakahara, Peter Colosi
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 93 Issue 2 Pg. 223-8 (Feb 2002) ISSN: 0910-5050 [Print] Japan
• PMID: 11856487 (Publication Type: Journal Article)

Chemical References

• Heat-Shock Proteins
• Interferon-beta

Topics

• Animals
• Brain Neoplasms (pathology, therapy)
• Dependovirus (genetics)
• Female
• Genetic Therapy
• Genetic Vectors
• Glioma (pathology, therapy)
• Heat-Shock Proteins (genetics)
• Humans
• Interferon-beta (genetics)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Transplantation
• Tumor Cells, Cultured