Effect of anti-P-selectin monoclonal antibody on renal ischemia/reperfusion injury in rats.

Abstract

OBJECTIVE:

To explore the effect of anti-P-selectin monoclonal antibody (mAb) on renal ischemia/reperfusion (I/R) injury in rats.

METHODS:

Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a renal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochemistry and Nested RT-PCR were used.

RESULTS:

Renal function insufficiency and renal histopathological damage were much less in the anti-P-selectin mAb-treated group than the saline-treated group. Plasma P-selectin levels were lower and renal P-selectin protein and mRNA expression were down-regulated in the former group.

CONCLUSION:

Anti-P-selectin mAb might be an efficient approach for the treatment of renal I/R injury.

• Authors: T Zhou, X Li, P Wu, D Zhang, M Zhang, N Chen, D Dong
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 113 Issue 9 Pg. 790-3 (Sep 2000) ISSN: 0366-6999 [Print] China
• PMID: 11776071 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• P-Selectin
• RNA, Messenger
• Creatinine

Topics

• Animals
• Antibodies, Monoclonal (pharmacology)
• Blood Urea Nitrogen
• Creatinine (blood)
• Gene Expression Regulation (drug effects)
• Kidney (blood supply, drug effects, pathology)
• Kidney Diseases (etiology, physiopathology, prevention & control)
• P-Selectin (blood, genetics, immunology)
• RNA, Messenger (drug effects, genetics, metabolism)
• Rats
• Rats, Wistar
• Reperfusion Injury (complications)
• Reverse Transcriptase Polymerase Chain Reaction