[Chronic hypoxia altered the mRNA expression of cyclooxygenase in intrapulmonary artery smooth muscle cell].

Abstract

OBJECTIVE:

To elucidate the effect of chronic hypoxia on the cyclooxygenase-2 mRNA expression in pulmonary artery smooth muscle cells of rats.

METHODS:

The gene expressions of COX-1 and COX-2 during acute hypoxia were measured with semi-quantified RT-PCR in the 2nd, 4th and 6th subculture of intrapulmonary artery smooth muscle cells (PASMC) of wistar rats under normoxic and continuous hypoxic condition separately.

RESULTS:

In all three generations, the expressions of COX-1 had no significant changes in hypoxia, while the COX-2 mRNA expression was higher in chronic hypoxic group than that in normoxic group and elevated with the duration of hypoxia exposure. After exposure to acute hypoxia, the augmentation of COX-2 mRNA expression in PASMC was higher in chronic hypoxic group than in normoxic group in all three subcultures.

CONCLUSION:

Chronic hypoxia could enhance the mRNA expression of cyclooxygenase-2 caused by acute hypoxia in PASMC of rats. It might be responsible for the lower HPV under chronic hypoxia.

• Authors: W Kong, D Wang
• Journal: Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases (Zhonghua Jie He He Hu Xi Za Zhi) Vol. 24 Issue 9 Pg. 531-3 (Sep 2001) ISSN: 1001-0939 [Print] China
• PMID: 11758166 (Publication Type: English Abstract, Journal Article)

Chemical References

• Isoenzymes
• Membrane Proteins
• RNA, Messenger
• Cyclooxygenase 1
• Cyclooxygenase 2
• Prostaglandin-Endoperoxide Synthases
• Ptgs1 protein, rat

Topics

• Animals
• Cell Hypoxia
• Cells, Cultured
• Cyclooxygenase 1
• Cyclooxygenase 2
• Gene Expression Regulation, Enzymologic
• Isoenzymes (genetics)
• Membrane Proteins
• Muscle, Smooth, Vascular (cytology, enzymology, metabolism)
• Prostaglandin-Endoperoxide Synthases (genetics)
• Pulmonary Artery (cytology, enzymology, metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Wistar