Laminin deposition to type IV collagen enhances haptotaxis, chemokinesis, and adhesion of hepatoma cells through beta1-integrins.
Abstract | BACKGROUND/AIMS: METHODS:
Hepatoma cell lines (KYN-1, -2 and -3) were used. The expression of integrin subunit mRNAs in hepatoma cells was confirmed by RT-PCR. The influence of laminin addition to type IV collagen on the adhesion, chemokinesis, and migration of KYN-1, -2 and -3 was evaluated by the haptotactic migration, phagokinetic track motility, and cell adhesion assays. The effects of integrin subunits on these activities were evaluated using the function- blocking antibodies for integrins. Phosphorylation of MEK1/2 and secretion of MMPs were investigated by Western blotting and gelatin zymography. RESULTS: CONCLUSIONS:
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Authors | T Torimura, T Ueno, M Kin, R Harad, T Nakamura, M Sakamoto, R Kumashiro, H Yano, M Kojiro, M Sata |
Journal | Journal of hepatology
(J Hepatol)
Vol. 35
Issue 2
Pg. 245-53
(Aug 2001)
ISSN: 0168-8278 [Print] Netherlands |
PMID | 11580147
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Collagen Type IV
- Integrin beta1
- Integrins
- Laminin
- RNA, Messenger
- RNA, Neoplasm
- MAP2K2 protein, human
- Protein-Tyrosine Kinases
- Protein Serine-Threonine Kinases
- MAP Kinase Kinase 1
- MAP Kinase Kinase 2
- MAP2K1 protein, human
- Mitogen-Activated Protein Kinase Kinases
- Matrix Metalloproteinases
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Topics |
- Carcinoma, Hepatocellular
(genetics, pathology, physiopathology)
- Cell Adhesion
- Cell Movement
- Collagen Type IV
(metabolism)
- Humans
- Integrin beta1
(genetics, metabolism)
- Integrins
(genetics, metabolism)
- Laminin
(metabolism)
- Liver Neoplasms
(genetics, pathology, physiopathology)
- MAP Kinase Kinase 1
- MAP Kinase Kinase 2
- Matrix Metalloproteinases
(metabolism)
- Mitogen-Activated Protein Kinase Kinases
(metabolism)
- Phosphorylation
- Protein Serine-Threonine Kinases
(metabolism)
- Protein-Tyrosine Kinases
(metabolism)
- RNA, Messenger
(genetics, metabolism)
- RNA, Neoplasm
(genetics, metabolism)
- Tumor Cells, Cultured
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