Abstract |
To examine the concordance of two metabolizing systems for use in genotoxocity testing with the micronucleus test, 15 naturally occurring substances ( arecoline, the plant extract aristolochic acid, beta-asarone, benzyl acetate, coumarin, emodine, isatidine dihydrate, monocrotaline, psoralen, reserpine, retrorsine, safrole, sanguinarine chloride, tannin and thiourea) were tested for their genotoxicity in the cytokinesis-block micronucleus test in vitro with human lymphocytes and in the presence and the absence of an exogenous metabolizing system from rat liver S9-mix and the metabolically competent human hepatoma cell line Hep-G2. Arecoline, the plant extract aristolochic acid, psoralen and tannin caused a significant increase in the number of micronuclei in human lymphocytes in the presence and the absence of an exogenous metabolising system from rat liver S9-mix and the metabolically competent human hepatoma cell line Hep-G2. A significant increase in the number of micronuclei with beta-asarone, coumarin, monocrotaline and retrorsine could be detected in the presence of S9-mix and the cell line Hep-G2. Benzyl acetate, emodine, isatidine dihydrate, reserpine, safrole, sanguinarine chloride and thiourea did not reveal any micronucleus inducing activity in either human lymphocytes or in Hep-G2. In addition to the other Hep-G2 results in the literature, this human hepatoma cell line could have a useful potential in the in vitro micronucleus test.
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Authors | S Kevekordes, J Spielberger, C M Burghaus, P Birkenkamp, B Zietz, P Paufler, M Diez, C Bolten, H Dunkelberg |
Journal | Anticancer research
(Anticancer Res)
2001 Jan-Feb
Vol. 21
Issue 1A
Pg. 461-9
ISSN: 0250-7005 [Print] Greece |
PMID | 11299780
(Publication Type: Journal Article)
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Chemical References |
- Biological Factors
- Biological Products
- Liver Extracts
- Mutagens
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Topics |
- Adult
- Animals
- Biological Factors
(toxicity)
- Biological Products
(toxicity)
- Carcinoma, Hepatocellular
- Dose-Response Relationship, Drug
- Humans
- Liver Extracts
(metabolism)
- Lymphocytes
(drug effects, ultrastructure)
- Micronucleus Tests
(methods)
- Mutagens
(toxicity)
- Rats
- Tumor Cells, Cultured
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