Abstract |
Water-soluble chitosan oligomer ( WSCO) has been reported to have anticancer activity, immuno-enhancing effect and antimicrobial activity. However, other biological activities are unknown. Herein, we have shown that WSCO is able to inhibit proliferation of human leukemia HL-60 cells and induce these cells to differentiate. Treatment with WSCO for 4 days resulted in a concentration-dependent reduction in HL-60 cell growth as measured by cell counting and MTT assay. This effect was accompanied by a marked increase in the proportion of G(0)/G(1) cells as measured by flow cytometry. WSCO also induced differentiation of the cells as measured by phorbol ester-dependent reduction of NBT, morphological changes as examined by Wright-Giemsa staining and expression of CD11b but not of CD14 as analysed by flow cytometry, indicating differentiation of HL-60 cells toward granulocyte-like cells. A combination of low dose of WSCO with all-trans retinoic acid, a differentiating agent toward granulocyte-like cells, exhibited a synergistic effect on the differentiation. In addition, treatment of HL-60 cells with WSCO for 6 or 8 days resulted in the induction of apoptosis as assayed qualitatively by agarose gel electrophoresis and quantitatively by Annexin V technique using flow cytometry. Collectively, there is a potential for WSCO in the treatment of myeloid leukemia.
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Authors | H O Pae, W G Seo, N Y Kim, G S Oh, G E Kim, Y H Kim, H J Kwak, Y G Yun, C D Jun, H T Chung |
Journal | Leukemia research
(Leuk Res)
Vol. 25
Issue 4
Pg. 339-46
(Apr 2001)
ISSN: 0145-2126 [Print] England |
PMID | 11248331
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- water soluble chitin
- Chitin
- Tretinoin
- Chitosan
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Cell Cycle
(drug effects)
- Cell Differentiation
(drug effects)
- Cell Division
(drug effects)
- Chitin
(analogs & derivatives, pharmacology)
- Chitosan
- Dose-Response Relationship, Drug
- Drug Synergism
- Granulocytes
(cytology)
- HL-60 Cells
(drug effects, pathology)
- Humans
- Leukemia, Promyelocytic, Acute
(drug therapy, pathology)
- Tretinoin
(pharmacology)
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