Abstract |
In this study we investigated the immunochemical and cytochemical reactivity of two monoclonal antibodies against the 16-amino acid tandem repeat of MUC4 to demonstrate a possible variation of the mucin core peptide expression related to lung cancer. The immunocytochemical anti-MUC4 reactivity was analyzed in four lung cancer cell lines (Calu-1, Calu-3, H460, SKMES) and in other tumor cell lines, as well as in frozen materials from 21 lung adenocarcinomas (ACs), including five bronchioloalveolar carcinomas (BACs), and 11 squamous cell lung carcinomas (SqCCs). A weak fluorescence anti-MUC4 positivity (range: 10.3-16.2) was observed only in acetone-fixed lung cancer cell lines Calu-1, Calu-3 and H460. These three lung cancer cell lines also showed a cytoplasmic immunoperoxidase reactivity. The immunostaining in lung cancer tissues showed a granular cytoplasmic reactivity: 15/21 (71%) and 17/21 (80%) ACs were positive with BC-LuC18.2 and BC-LuCF12, respectively. All BACs were positive. Moderate to strong reactivity was present in well-differentiated ACs. In the normal lung parenchyma counterparts weak reactivity was found only in bronchiolar cells. All SqCCs were negative. Anti-MUC4 reactivity was also observed in the alveolar mucus. In conclusion, our anti-MUC4 MAbs detect a secretion product present in mucus and this product is elaborated by lung cancer cells and overexpressed in well-differentiated lung ACs.
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Authors | C Botti, E Seregni, S Ménard, P Collini, E Tagliabue, M Campiglio, B Vergani, C Ghirelli, P Aiello, S Pilotti, E Bombardieri |
Journal | The International journal of biological markers
(Int J Biol Markers)
2000 Oct-Dec
Vol. 15
Issue 4
Pg. 312-20
ISSN: 0393-6155 [Print] United States |
PMID | 11192827
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, Neoplasm
- MUC4 protein, human
- Muc4 protein, mouse
- Mucin-4
- Mucins
- Neoplasm Proteins
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Topics |
- Adenocarcinoma
(immunology, pathology)
- Adenocarcinoma, Bronchiolo-Alveolar
(immunology, pathology)
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antigens, Neoplasm
(immunology)
- Carcinoma, Squamous Cell
(immunology, pathology)
- Female
- Flow Cytometry
- Fluorescent Antibody Technique
- Humans
- Lung Neoplasms
(immunology, pathology)
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Mucin-4
- Mucins
(immunology)
- Neoplasm Proteins
(immunology)
- Tandem Repeat Sequences
- Tumor Cells, Cultured
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