Dendritic cells permit identification of genes encoding MHC class II-restricted epitopes of transplantation antigens.

Abstract	Minor or histocompatibility (H) antigens are recognized by CD4+ and CD8+ T lymphocytes as short polymorphic peptides associated with MHC molecules. They are the targets of graft versus host and graft versus leukemia responses following bone marrow transplantation between HLA-identical siblings. Several genes encoding class I-restricted minor H epitopes have been identified, but approaches used for these have proved difficult to adapt for cloning class II-restricted minor H genes. We have combined the unique antigen-presenting properties of dendritic cells and high levels of episomal expression following transfection of COS cells to identify a Y chromosome gene encoding two HY peptide epitopes, HYAb and HYEk.
Authors	D Scott, C Addey, P Ellis, E James, M J Mitchell, N Saut, S Jurcevic, E Simpson
Journal	Immunity (Immunity) Vol. 12 Issue 6 Pg. 711-20 (Jun 2000) ISSN: 1074-7613 [Print] United States
PMID	10894170 (Publication Type: Journal Article)
Chemical References	Epitopes, T-Lymphocyte Eukaryotic Initiation Factor-2 H-2 Antigens H-2A antigen H-Y Antigen Histocompatibility Antigens Minor Histocompatibility Antigens Nuclear Proteins Peptides Proteins UTY protein, human Uty protein, mouse Histone Demethylases Jarid1d protein, mouse DDX3Y protein, human Ddx3y protein, mouse DEAD-box RNA Helicases
Topics	Amino Acid Sequence Animals DEAD-box RNA Helicases Dendritic Cells (immunology, metabolism) Epitopes, T-Lymphocyte (biosynthesis, genetics, immunology) Eukaryotic Initiation Factor-2 (genetics) Female Gene Expression Regulation (immunology) Genes, MHC Class II H-2 Antigens (genetics) H-Y Antigen (biosynthesis, genetics) Histocompatibility Antigens (biosynthesis, genetics, immunology) Histone Demethylases Immunity, Innate (genetics) Mice Mice, Inbred C57BL Mice, Inbred CBA Mice, Transgenic Minor Histocompatibility Antigens (genetics) Molecular Sequence Data Nuclear Proteins Peptides (genetics, immunology) Proteins (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!