Constitutive expression and regulation of collagenase-3 in human breast cancer cells.

Abstract

Matrix metalloproteinases (MMPs) are a family of secreted or transmembrane proteins that have been implicated in multiple physiological and pathological processes related to extracellular matrix turnover. Recent evidence strongly suggests a role for collagenase-3 (MMP-13) in tumor metastasis and invasion. We report here that collagenase-3 is constitutively expressed in the breast cancer cell line MDA-MB231 (MDA) and outline the molecular mechanism regulating its expression. Functional analysis of the collagenase-3 promoter showed that both the activator protein-1 (AP-1) site and the runt domain (RD) binding site were required for maximal constitutive expression of collagenase-3 in MDA cells. Determination of factors binding to those sites by Northern analysis and transient transfections identified the requirement of Fra-1, c-Jun, and Cbfa1 for basal collagenase-3 promoter activity in MDA cells.

• Authors: N Selvamurugan, N C Partridge
• Journal: Molecular cell biology research communications : MCBRC (Mol Cell Biol Res Commun) Vol. 3 Issue 4 Pg. 218-23 (Apr 2000) ISSN: 1522-4724 [Print] United States
• PMID: 10891395 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright 2000 Academic Press.

Chemical References

• Core Binding Factor Alpha 1 Subunit
• DNA-Binding Proteins
• Neoplasm Proteins
• Proto-Oncogene Proteins
• Proto-Oncogene Proteins c-fos
• RNA, Messenger
• RUNX1 Translocation Partner 1 Protein
• RUNX1T1 protein, human
• Transcription Factor AP-1
• Transcription Factors
• fos-related antigen 1
• Collagenases
• MMP13 protein, human
• Matrix Metalloproteinase 13

Topics

• Breast Neoplasms (enzymology, genetics, metabolism, pathology)
• Collagenases (genetics, metabolism)
• Core Binding Factor Alpha 1 Subunit
• DNA-Binding Proteins (genetics, metabolism)
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Neoplastic
• Genes, Reporter
• Genes, jun (genetics)
• Humans
• Matrix Metalloproteinase 13
• Neoplasm Proteins
• Promoter Regions, Genetic (genetics)
• Proto-Oncogene Proteins
• Proto-Oncogene Proteins c-fos (genetics)
• RNA, Messenger (genetics, metabolism)
• RUNX1 Translocation Partner 1 Protein
• Response Elements (genetics)
• Transcription Factor AP-1 (genetics, physiology)
• Transcription Factors (genetics, metabolism)
• Transfection
• Tumor Cells, Cultured