Abstract | BACKGROUND & AIMS: Because alterations of integrin expression in cancers contribute to cancer cell biology, we analyzed the association between the potential for peritoneal dissemination and integrin expression. METHODS: RESULTS: The level of integrin beta4 subunit expression was inversely correlated with dissemination potential. Introduction of a full-length complementary DNA ( cDNA) for beta4 subunit into cancer cells showing negligible beta4 subunit expression markedly suppressed peritoneal dissemination and inhibition of endogenous integrin alpha6beta4 by introduction of a cytoplasmic domain-deleted beta4 subunit cDNA into cells showing high expression of beta4 subunit promoted peritoneal dissemination. Apoptosis, which was histologically evident in peritoneal nodules of SCID mice, was induced in the cells with high beta4 subunit expression by attachment to laminin and stimulation with growth factors in vitro. An immunohistochemical study of specimens from 120 cases of primary gastric cancer showed that patients with beta4 subunit-positive tumors exhibited peritoneal dissemination only infrequently (P < 0.0001) and had a better outcome (P < 0.01). CONCLUSIONS:
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Authors | Y Ishii, A Ochiai, T Yamada, S Akimoto, K Yanagihara, M Kitajima, S Hirohashi |
Journal | Gastroenterology
(Gastroenterology)
Vol. 118
Issue 3
Pg. 497-506
(Mar 2000)
ISSN: 0016-5085 [Print] United States |
PMID | 10702200
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Surface
- Biomarkers
- DNA, Complementary
- Integrin alpha6beta4
- Integrins
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Topics |
- Animals
- Antigens, Surface
(genetics, physiology)
- Apoptosis
(physiology)
- Biomarkers
- Cytoplasm
(genetics)
- DNA, Complementary
(genetics)
- Forecasting
- Gene Deletion
- Humans
- Integrin alpha6beta4
- Integrins
(genetics, physiology)
- Mice
- Mice, SCID
- Neoplasm Invasiveness
(pathology, prevention & control)
- Peritoneum
(pathology)
- Stomach Neoplasms
(pathology)
- Tumor Cells, Cultured
(metabolism)
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