Abstract |
The current study aimed to investigate the protective effect of the cardenolide glycoside acovenoside A (AcoA) against doxorubicin-induced cardiotoxicity in mice. AcoA was isolated from the pericarps of Acokanthera oppositifolia to chemical homogeneity and characterized by means of one- and two-dimensional nuclear magnetic resonance spectroscopy. AcoA exhibited relatively low toxicity in mice (LD50 = 223.3 mg/kg bw). Repeated administration of doxorubicin induced cardiotoxicity manifested by reduced activity of myocardial membrane-bound ion pumps and elevated serum biomarkers of myocardial dysfunction, oxidative stress, and inflammation. Pretreatment of doxorubicin-exposed mice with AcoA (11.16 or 22.33 mg/kg bw, i.p.) for 2 weeks after 2 weeks of combined administration of AcoA and doxorubicin protected the animals dose dependently against doxorubicin-induced cardiotoxicity as indicated by normalization of the levels of different myocardial markers of oxidative stress ( malondialdehyde, nitric oxide, total antioxidant capacity, and cardiac glutathione), serum myocardial diagnostic marker enzymes (serum cardiac troponin T, creatine kinase isoenzyme MB, aspartate aminotransferase, and lactate dehydrogenase), and inflammatory markers ( c-reactive protein, tumor necrosis factor-α, and interleukin-6), as well as myocardial Na(+)/K(+)- ATPase activity. These effects were attributed to the negative impact of AcoA on transcription factors nuclear factor κB and interferon regulatory factor 3/7. Thus acovenoside A might act as a cardioprotective agent to prevent doxorubicin-induced cardiotoxicity.
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Authors | Shahira M Ezzat, Menna El Gaafary, Abeer M El Sayed, Omar M Sabry, Zeinab Y Ali, Susanne Hafner, Michael Schmiech, Lu Jin, Tatiana Syrovets, Thomas Simmet |
Journal | The Journal of pharmacology and experimental therapeutics
(J Pharmacol Exp Ther)
Vol. 358
Issue 2
Pg. 262-70
(08 2016)
ISSN: 1521-0103 [Electronic] United States |
PMID | 27247000
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 by The American Society for Pharmacology and Experimental Therapeutics. |
Chemical References |
- Biomarkers
- Cardenolides
- Cardiotonic Agents
- Interferon Regulatory Factors
- NF-kappa B
- acovenoside A
- Doxorubicin
- DNA Topoisomerases, Type II
- Sodium-Potassium-Exchanging ATPase
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Topics |
- Animals
- Apocynaceae
(chemistry)
- Biocatalysis
- Biomarkers
(blood)
- Cardenolides
(chemistry, isolation & purification, pharmacology)
- Cardiotonic Agents
(chemistry, isolation & purification, pharmacology)
- Cardiotoxicity
(etiology, metabolism, pathology, prevention & control)
- DNA Topoisomerases, Type II
(metabolism)
- Doxorubicin
(adverse effects)
- Interferon Regulatory Factors
(metabolism)
- Male
- Mice
- Mitochondrial Membranes
(drug effects, enzymology)
- Models, Molecular
- Molecular Conformation
- Myocardium
(metabolism, pathology)
- NF-kappa B
(metabolism)
- Oxidative Stress
(drug effects)
- Sodium-Potassium-Exchanging ATPase
(metabolism)
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