Abstract | BACKGROUND: Although, Dan-Lou prescription (DLP) is used for antagonizing check discomfort and heartache, the pharmacological mechanism has not been clearly illustrated. Our present study aimed to design inflammatory models induced by LPS in vivo and in vitro to investigate the anti- inflammation of DLP ethanol extract (EEDL) and the potential mechanisms. METHODS: RESULTS: Eight compounds from EEDL have been identified as gallic acid, salvianic acid, puerarin, daidzin, paeoniflorin, salvianolic acid B, cryptotanshinone, and tanshinone IIA, with amounts of 0.26, 9.84, 10.41, 2.55, 9.44, 3.82, 0.24 and 0.3 mg/kg, respectively. In vivo, EEDL administration antagonized the up-regulation of more than 17 kinds of cytokines, chemokines and acute phase proteins in LPS-treated mice serum, and inhibited LPS-induced IL-6 mRNA and protein expression in mice liver tissue. In vitro, LPS-induced NO and PGE2 over-productions were decreased by EEDL treatment. The mRNA and protein expression of iNOS, COX-2 and IL-6 were similarly inhibited by EEDL treatment, which might be attributed to decrease the DNA-binding activity of NF-κB p65. CONCLUSION: EEDL was valid for anti- inflammation and the potential molecular mechanisms might be due to the inhibition of of LPS-induced iNOS/NO, COX-2/ PGE2 and cytokines expression by antagonizing the activation of NF-κB p65.
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Authors | Li-Na Gao, Xin Zhou, Yi Zhang, Yuan-Lu Cui, Chun-Quan Yu, Shan Gao |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 15
Pg. 317
(Sep 09 2015)
ISSN: 1472-6882 [Electronic] England |
PMID | 26354089
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents
- Cytokines
- Drugs, Chinese Herbal
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Topics |
- Animals
- Anti-Inflammatory Agents
(chemistry, pharmacology)
- Cell Line
- Cytokines
(analysis, metabolism)
- Drugs, Chinese Herbal
(chemistry, pharmacology)
- Mice
- Up-Regulation
(drug effects)
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