Curcumin improves hypoxia induced dysfunctions in 3T3-L1 adipocytes by protecting mitochondria and down regulating inflammation.

Abstract

Obesity induced metabolic syndrome is increasing worldwide at an alarming rate. It is characterized by excessive expansion of white adipose tissue which leads to hypoxia and impairs normal metabolism. Recent studies reveal that hypoxia could be one of the factors for inflammation, insulin resistance and other obesity related complications. There is a high demand for anti-obese phytoceuticals to control and manage the complications resulting from obesity. In this study, we investigated how hypoxia affect the physiological functions of 3T3-L1 adipocytes emphasizing on oxidative stress, inflammation, and mitochondrial functions. We also evaluated the protective role of various doses of curcumin, a well-known dietary antioxidant, on hypoxia induced alterations. The results revealed that hypoxia significantly altered the vital parameters of adipocyte biology like HIF 1α expression (103.47% ↑), lactate, and glycerol release (184.34% and 69.1% ↑, respectively), reactive oxygen species production (432.53% ↑), lipid and protein oxidation (376.6% and 566.6% ↑, respectively), reduction in antioxidant enzymes (superoxide dismutase and catalase) status, secretion of inflammatory markers (TNF α, IL 6, IL 1β, and IFN γ), and mitochondrial functions (mitochondrial mass, membrane potential, permeability transition pore integrity, and superoxide generation). Curcumin substantially protected adipocytes from toxic effects of hypoxia in a dose dependent manner by protecting mitochondria and down regulating inflammation. Acriflavine is used as a positive control. A detailed investigation is required for the development of curcumin as an effective nutraceutical against obesity.

• Authors: Ariyapalli Priyanka, Sasidharan Suseela Anusree, Vijayakumar Marykutty Nisha, Kozhiparambil Gopalan Raghu
• Journal: BioFactors (Oxford, England) (Biofactors) 2014 Sep-Oct Vol. 40 Issue 5 Pg. 513-23 ISSN: 1872-8081 [Electronic] Netherlands
• PMID: 25110893 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 International Union of Biochemistry and Molecular Biology.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Cytokines
• Hif1a protein, mouse
• Hypoxia-Inducible Factor 1, alpha Subunit
• Mitochondrial Membrane Transport Proteins
• Mitochondrial Permeability Transition Pore
• Lactic Acid
• Curcumin

Topics

• 3T3-L1 Cells
• Adipocytes
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Cell Hypoxia
• Curcumin (pharmacology)
• Cytokines (metabolism)
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Lactic Acid (metabolism)
• Lipid Peroxidation
• Membrane Potential, Mitochondrial
• Mice
• Mitochondria (drug effects, metabolism)
• Mitochondrial Membrane Transport Proteins (metabolism)
• Mitochondrial Permeability Transition Pore
• Obesity (drug therapy, metabolism)
• Oxidative Stress
• Protein Carbonylation