Immunological characterization of a neuronal antibody (anti-Tr) associated with paraneoplastic cerebellar degeneration and Hodgkin's disease.

Abstract

We studied an autoantibody (called anti-Tr), found in the serum and CSF of five patients with paraneoplastic cerebellar degeneration (PCD) and Hodgkin's disease (HD). Anti-Tr antibodies labelled the cytoplasm of Purkinje cells of human and rat cerebellum. The molecular layer of rat cerebellum showed a characteristic dotted pattern suggestive of immunoreactivity of dendritic spines of Purkinje cells. Patients with cerebellar disorders without HD (159) or HD without PCD (30) did not harbor anti-Tr antibodies. Immunoblots of human Purkinje cells or rat and mouse cerebellum were negative. Anti-Tr antibodies, as defined in this study, appear specific for HD-associated PCD. The immunohistochemical pattern described in the rat cerebellum coupled with the absence of reactivity in the immunoblot may be used to identify anti-Tr antibodies.

• Authors: F Graus, J Dalmau, F Valldeoriola, I Ferrer, R Reñe, C Marin, C J Vecht, T Arbizu, C Targa, J W Moll
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 74 Issue 1-2 Pg. 55-61 (Apr 1997) ISSN: 0165-5728 [Print] Netherlands
• PMID: 9119979 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies

Topics

• Adolescent
• Adult
• Aged
• Animals
• Antibodies (analysis, cerebrospinal fluid, immunology)
• Cerebellar Diseases (complications, immunology)
• Female
• Hodgkin Disease (cerebrospinal fluid, complications, immunology)
• Humans
• Immunoblotting
• Immunohistochemistry
• Male
• Mice
• Mice, Inbred BALB C
• Nerve Degeneration
• Neurons (immunology)
• Paraneoplastic Syndromes (cerebrospinal fluid, complications, immunology)
• Rats
• Rats, Wistar
• Tissue Distribution