[Quantification of feline immunodeficiency virus (FIV) RNA in the plasma of infected cats].

Abstract

A competitive reverse transcription-polymerase chain reaction (RT-PCR) was used to quantify RNA of feline immunodeficiency virus (FIV) in cats. The assay uses in vitro synthesized RNA as a competitive internal control. The synthesized RNA has a 22-base deletion with respect to the wild-type sequence. PCR products were quantitated by densitometric analysis of a digitized image of the ethidium bromide stained gel. Viral RNA concentrations in the plasma of two cats experimentally infected with FIV strain UT113 were followed for 32 weeks; peak copy numbers (2.3 x 10(4) and 1.3 x 10(4) per ml, respectively) were reached 11 weeks after subcutaneous injection of ten 50% cat infectious doses. With rising antibody titers against FIV-gag and FIV-env gene products, the amount of FIV RNA in plasma decreased. Nine asymptomatic cats that had been experimentally infected 3.5 to 4.5 years earlier had copy numbers between 5.6 x 10(3) and 4.3 x 10(4) per ml. Cats treated for six weeks with 9-(2-phosphonylmethoxy-propyl)-2,6-diaminopurine [PMPDAP] (20 mg/kg body weight s.c. three times a week) showed a significant decrease of RNA copy numbers in plasma. This quantitative competitive RT-PCR will be useful to study the pathogenesis of the FIV infection, to evaluate the effectiveness of vaccines and to monitor antiviral and immunomodulating drugs.

• Authors: T W Vahlenkamp, A De Ronde, M C Horzinek, H F Egberink
• Journal: Berliner und Munchener tierarztliche Wochenschrift (Berl Munch Tierarztl Wochenschr) Vol. 109 Issue 8 Pg. 265-9 (Aug 1996) ISSN: 0005-9366 [Print] Germany
• Vernacular Title: Quantifizierung feliner Immunschwächevirus (FIV)-RNS im Plasma infizierter Katzen.
• PMID: 9005834 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• RNA, Viral

Topics

• Animals
• Antibodies, Viral (blood)
• CD4-CD8 Ratio
• Cats
• Feline Acquired Immunodeficiency Syndrome (virology)
• Immunodeficiency Virus, Feline (genetics, isolation & purification)
• Polymerase Chain Reaction (veterinary)
• RNA, Viral (blood)
• Viremia (virology)