Mitochondrial damage following exposure of organotypic cultures of human malignant gliomas to 2-chloro- and 2-bromo-2'-deoxyadenosine.

Abstract

The effects of 2-chloro-2'-deoxyadenosine (cladribine, 2-CdA) and a closely related compound 2-bromo-2'-deoxyadenosine (2-BdA) on organotypic cultures of human malignant gliomas were studied with the use of electron microscopy. The cultures grown from surgical biopsies included six glioblastomas, three anaplastic astrocytomas and low-grade fibrillary astrocytoma. After 6 to 10 days of the in vitro growth the cultures were exposed to 0.3-10 microM 2-CdA or 2-BdA for 1 to 10 days. Mitochondrial swelling and disappearance of cristae following exposure to the tested substances were observed, but only in highly anaplastic (low-differentiated) tumor cells. The mitochondrial toxicity was dose- and time-dependent, and no difference was found between the effect of 2-CdA and 2-BdA.

• Authors: E Matyja, P Grieb, M Ryba, J Jagielski, M J Mossakowski
• Journal: Folia neuropathologica (Folia Neuropathol) Vol. 34 Issue 1 Pg. 31-9 ( 1996) ISSN: 1641-4640 [Print] Poland
• PMID: 8855085 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Cladribine

Topics

• Antineoplastic Agents (adverse effects, pharmacology)
• Astrocytoma (pathology, ultrastructure)
• Cell Movement
• Cladribine (adverse effects, pharmacology)
• Culture Techniques
• Glioblastoma (pathology, ultrastructure)
• Glioma (pathology, ultrastructure)
• Humans
• Mitochondria (drug effects, pathology)