Abstract | PROBLEM: METHODS:
DNA was extracted from whole blood samples of 1) 51 couples with at least three miscarriages, and 2) 43 fertile couples (with at least seven children and no known history of recurrent miscarriage). The polymerase chain reaction (PCR) was used to amplify the second exon of the HLA DQ alpha locus on chromosome 6. Genotypes were identified by allele specific hybridization with 12 sequence-specific oligonucleotide probes. RESULTS: CONCLUSIONS: Reproductive partners with unexplained recurrent pregnancy loss have no increased frequency of HLA DQ alpha allele sharing. It is unlikely that HLA DQ alpha genotyping will be helpful in the management of patients with RSA.
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Authors | D Dizon-Townson, L Nelson, J R Scott, D W Branch, K Ward |
Journal | American journal of reproductive immunology (New York, N.Y. : 1989)
(Am J Reprod Immunol)
Vol. 34
Issue 4
Pg. 209-12
(Oct 1995)
ISSN: 1046-7408 [Print] Denmark |
PMID | 8579756
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Abortion, Habitual
(genetics)
- Adult
- Alleles
- Female
- Fertility
(genetics)
- Gene Frequency
- Genotype
- HLA-DQ Antigens
(genetics)
- Humans
- Male
- Polymerase Chain Reaction
- Pregnancy
- Spouses
(statistics & numerical data)
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