Abstract |
We have found that antithymocyte globulin (ATG), an equine antibody with proven efficacy in aplastic anaemia (AA), has a direct stimulatory effect on primitive haemopoietic cells from normal donors. This growth stimulation may be mediated via anti-CD45RO activity present in the ATG preparation. Addition of unabsorbed ATG enhanced colony growth at 21 d in the blast colony forming cell (Bl-CFC) assay. Prior absorption of ATG by incubation with the CD45RO+ MOLT-4 cell line resulted in the loss of enhancement. Absorption by MOLT-4 cells preincubated with anti-CD45RO mAb, UCHL-1, restored ATG's stimulatory effect. The Bl-CFC could also be stimulated to grow by the addition of UCHL-1 directly. Incubation of the primitive haemopoietic cells for 4 h with ATG was associated with a decline in the antigenic density of CD45RO, a tyrosine phosphatase. This down-regulation may upset the balance between growth factor-induced tyrosine kinase activation and tyrosine phosphate dephosphorylation resulting in increased growth of primitive cells, a possible factor in the sustained recovery of haemopoiesis seen in AA patients after ATG treatment.
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Authors | A T Huang, N G Mold |
Journal | British journal of haematology
(Br J Haematol)
Vol. 88
Issue 3
Pg. 643-6
(Nov 1994)
ISSN: 0007-1048 [Print] England |
PMID | 7819083
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antilymphocyte Serum
- Leukocyte Common Antigens
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Topics |
- Anemia, Aplastic
(blood, immunology, pathology)
- Antilymphocyte Serum
(immunology, therapeutic use)
- Cell Division
- Flow Cytometry
- Hematopoietic Stem Cells
(immunology, pathology, physiology)
- Humans
- Leukocyte Common Antigens
(immunology, physiology)
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