Evaluation of
albumin estimation by
bromocresol green (BCG) method was carried out in sixty nephrotics and twenty control subjects. In
nephrotic syndrome, varies; is directly proportional, to 2-globulin and total
cholesterol concentrations were significantly increased, while the mean
albumin level was significantly reduced when compared with the corresponding control values. In both control and nephrotics, the determination of
serum albumin by the BCG method showed good correlations with values obtained by
cellulose acetate electrophoresis using the
biuret method to determine the total
protein, but the mean value for the nephrotics was higher by an average of 0.4g/100ml. Interference with the BCG reaction by an increased varies; is directly proportional, to 2-globulin level was suggested as a possible explanation for the higher mean
albumin level obtained by the BCG method in the nephrotics. Inclusion of 0.8M NaCl in the BCG assay system did not prevent the interference by other
proteins. However, this interference could to a large extent, be offset by calibrating with a pool of fresh sera previously determined by electrophoresis.