Two-step freezing of two-cell rabbit embryos after partial dehydration at room temperature.
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| Abstract |
The effect of rapid freezing and thawing on the survival of 2-cell rabbit embryos was examined. When embryos in 2.2 M-propanediol were directly plunged from room temperature to liquid nitrogen some of them survived after thawing (8%) but only if they had been pretreated by exposure to an impermeable solute, sucrose, that makes the blastomeres shrink osmotically before cooling. High survival (77-88%) in vitro was obtained when pretreated embryos were first held at -30 degrees C for 30-240 min before immersion into liquid nitrogen. Transfer of such frozen-thawed embryos gave a survival rate to live young similar to that obtained with controls (26% and 32% respectively). DMSO was less effective than propanediol; only 2 out of 38 sucrose-pretreated frozen-thawed embryos developed in vitro. The present work shows that a combination of partial dehydration of blastomeres at room temperature with their permeation by a cryoprotective agent offers a simple method for successful rapid freezing and thawing of rabbit embryos.
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| Authors | J P Renard, Bui-Xuan-Nguyen, V Garnier |
| Journal | Journal of reproduction and fertility (J Reprod Fertil) Vol. 71 Issue 2 Pg. 573-80 (Jul 1984) ISSN: 0022-4251 [Print] England |
| PMID | 6747965 (Publication Type: Journal Article) |
| Chemical References |
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