Mature methyl-deficient tRNA isolated from a mammary adenocarcinoma.

Abstract

A transplantable rat tumor, mammary adenocarcinoma 13762, accumulates tRNA which can be methylated in vitro by mammalian tRNA (adenine-1) methyltransferase. This unusual ability of the tumor RNA to serve as substrate for a homologous tRNA methylating enzyme is correlated with unusually low levels of the A 58-specific adenine-1 methyltransferase. The nature of the methyl-accepting RNA has been examined by separating tumor tRNA on two-dimensional polyacrylamide gels. Comparisons of ethidium bromide-stained gels of tumor vs. liver tRNA show no significant quantitative differences and no accumulation of novel tRNAs or precursor tRNAs in adenocarcinoma RNA. Two-dimensional separations of tumor RNA after in vitro [14C]methylation using purified adenine-1 methyltransferase indicate that about 25% of the tRNA species are strongly methyl-accepting RNAs. Identification of six of the tRNAs separated on two-dimensional gels has been carried out by hybridization of cloned tRNA genes to Northern blots. Three of these, tRNALys3 , tRNAGln and tRNAMeti , are among the adenocarcinoma methyl-accepting RNAs. The other three RNAs, all of which are leucine-specific tRNAs, show no methyl-accepting properties. Our results suggest that low levels of a tRNA methyltransferase in the adenocarcinoma cause selected species of tRNA to escape the normal A58 methylation, resulting in the appearance of several mature tRNAs which are deficient in 1-methyladenine. The methyl-accepting tRNAs from the tumor appear as ethidium bromide-stained spots of similar intensity to those seen for RNA from rat liver; therefore, methyladenine deficiency does not seem to impair processing of these tRNAs.

• Authors: P S Leboy, B D Uschmann, C E Salas
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 782 Issue 2 Pg. 156-63 (Jun 16 1984) ISSN: 0006-3002 [Print] Netherlands
• PMID: 6722163 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Tritium
• tRNA Methyltransferases
• tRNA (adenine(58)-N(1))-methyltransferase

Topics

• Adenocarcinoma (enzymology)
• Animals
• Cell Line
• Electrophoresis, Polyacrylamide Gel (methods)
• Female
• Liver (analysis)
• Mammary Neoplasms, Experimental (enzymology)
• Methylation
• Rats
• Rats, Inbred F344
• Tritium
• tRNA Methyltransferases (deficiency, metabolism)