Inhibition of dopamine beta-hydroxylase by 4-hydroxypyrazole: ethanol-pyrazole effects on serum dopamine beta-hydroxylase in vivo.

Abstract

Chronic pyrazole treatment caused a 40% decrease in rat serum dopamine beta-hydroxylase (DBH) activity. Ethanol given simultaneously with pyrazole prevented the inhibition. 4-Hydroxypyrazole, the major metabolite of pyrazole, competitively inhibited both rat serum DBH and partially purified bovine adrenal DBH in vitro. In vivo, 4-hydroxypyrazole caused large decreases in rat serum (53-84%) and adrenal (97%) DBH activity but had no effect on brain enzyme. The decrease in rat serum DBH after chronic pyrazole treatment and the pyrazole-induced changes in physiological parameters, such as body weight and temperature, may be due to the formation of 4-hydroxypyrazole.

• Authors: J D Harralson, B I Wolfe, F C Brown
• Journal: The Journal of pharmacology and experimental therapeutics (J Pharmacol Exp Ther) Vol. 206 Issue 1 Pg. 69-74 (Jul 1978) ISSN: 0022-3565 [Print] United States
• PMID: 660559 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Pyrazoles
• Ethanol
• Dopamine beta-Hydroxylase

Topics

• Adrenal Glands (drug effects, enzymology)
• Animals
• Cattle
• Dopamine beta-Hydroxylase (antagonists & inhibitors, blood)
• Ethanol (pharmacology)
• Hydroxylation
• In Vitro Techniques
• Male
• Pyrazoles (antagonists & inhibitors, pharmacology)
• Rats