Radioimmunoassay of cationic trypsin-like immunoreactivity in man.

Abstract

The present paper describes the preparation of human cationic trypsin and of a stable and fully immunoreactive 125I-labelled tosyl-lysine chloromethyl ketone (TLCK) cationic trypsin by a modified chloramine-T method with a high specific radioactivity; the production of an avid and specific rabbit cationic trypsin antiserum; and a sensitive, precise, and specific radioimmunoassay method enabling measurements of fasting serum trypsin-like immunoreactivity (CTLI) in the low ng/ml range in normals; the significant rise in serum CTLI in patients with normal pancreatic exocrine secretion; and the absence of any rise in patients with severely reduced pancreatic exocrine secretion after intravenous injection of secretion; a markedly elevated fasting serum CTLI level in patients with acute pancreatitis; fasting CTLI in duodenal juice; and separation of CTLI in one minor and one major molecular component in fasting serum.

• Authors: J Florholmen, R Jorde, R L Olsen, S Kolmannskog, P G Burhol
• Journal: Scandinavian journal of gastroenterology (Scand J Gastroenterol) Vol. 19 Issue 5 Pg. 613-21 (Jul 1984) ISSN: 0036-5521 [Print] England
• PMID: 6474083 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Iodine Radioisotopes
• Tosyllysine Chloromethyl Ketone
• Trypsin

Topics

• Duodenum (analysis)
• Electrophoresis, Polyacrylamide Gel
• Humans
• Immunoelectrophoresis
• Intestinal Secretions (analysis)
• Iodine Radioisotopes
• Radioimmunoassay (methods)
• Tosyllysine Chloromethyl Ketone
• Trypsin (immunology, metabolism)