Repression of rearranged mu gene and translocated c-myc in mouse 3T3 cells X Burkitt lymphoma cell hybrids.

Abstract

The productively rearranged immunoglobulin mu chain gene and the translocated cellular oncogene c-myc are transcribed at high levels both in human Burkitt lymphoma cells carrying the t(8;14) chromosome translocation and in mouse plasmacytoma X Burkitt lymphoma cell hybrids. In the experiments reported here these genes were found to be repressed in mouse 3T3 fibroblast X Burkitt lymphoma cell hybrids. Such repression probably occurs at the transcriptional level since no human mu- and c-myc messenger RNA's are detectable in hybrid clones carrying the corresponding genes. It is therefore concluded that the ability to express these genes requires a differential B cell environment. The results suggest that the 3T3 cell assay may not be suitable to detect oncogenes directly involved in human B cell oncogenesis, since 3T3 cells apparently are incapable of transcribing an oncogene that is highly active in malignant B cells with specific chromosomal translocations.

• Authors: K Nishikura, A ar-Rushdi, J Erikson, E DeJesus, D Dugan, C M Croce
• Journal: Science (New York, N.Y.) (Science) Vol. 224 Issue 4647 Pg. 399-402 (Apr 27 1984) ISSN: 0036-8075 [Print] United States
• PMID: 6424234 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Immunoglobulin Heavy Chains
• Immunoglobulin mu-Chains
• RNA, Messenger

Topics

• Animals
• Burkitt Lymphoma (genetics)
• Fibroblasts
• Gene Expression Regulation
• Genes
• Humans
• Hybrid Cells (metabolism)
• Immunoglobulin Heavy Chains (genetics)
• Immunoglobulin mu-Chains (genetics)
• Mice
• Oncogenes
• RNA, Messenger (genetics)
• Transcription, Genetic
• Translocation, Genetic